Inhibition of bacterial DNA-dependent RNA polymerases and restriction endonuclease by UV-absorbing components from propolis

Several UV-absorbing substances inhibiting the DNA-dependent RNA polymerases of Escherichia coli and Streptomyces aureofaciens, as well as the restriction endonuclease Eco RI have been isolated from the water-soluble extract of Propolis by two-dimensional paper chromatography. The inhibition of bact...

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Bibliographic Details
Published inPharmazie Vol. 41; no. 2; p. 131
Main Authors Simúth, J, Trnovský, J, Jeloková, J
Format Journal Article
LanguageEnglish
Published Germany 01.02.1986
Subjects
Chromatography, Paper
Deoxyribonuclease I - analysis
DNA Restriction Enzymes - antagonists & inhibitors
DNA, Viral - analysis
DNA-Directed RNA Polymerases - antagonists & inhibitors
Electrophoresis, Polyacrylamide Gel
Escherichia coli - enzymology
Pancreas - enzymology
Propolis - analysis
Propolis - pharmacology
Resins, Plant - pharmacology
Spectrophotometry, Ultraviolet
Transcription, Genetic - drug effects
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Summary:Several UV-absorbing substances inhibiting the DNA-dependent RNA polymerases of Escherichia coli and Streptomyces aureofaciens, as well as the restriction endonuclease Eco RI have been isolated from the water-soluble extract of Propolis by two-dimensional paper chromatography. The inhibition of bacterial RNA-polymerases by the components of Propolis was probably due to the loss of their ability to bind to DNA. The general characteristic of the UV-absorbing component of Propolis with the most pronounced inhibitory effect upon transcription in vitro is described.
ISSN:0031-7144