Single-isotope enzymatic derivative method for measuring catecholamines in human plasma

• Published in: Journal of clinical chemistry and clinical biochemistry Vol. 18; no. 7; p. 413
• Main Authors: Bosak, J, Knoll, E, Ratge, D, Wisser, H
• Format: Journal Article
• Language: English
• Published: Germany 01.07.1980
• Subjects: Animals; Blood Chemical Analysis - methods; Catechol O-Methyltransferase; Catecholamines - blood; Methionine Adenosyltransferase; Rats; Tritium

The radioenzymatic determination of plasma catecholamines with a modification of the method of da Prada & Zürcher ((1976), Life Sci 19, 1161-1174) is described. The several reaction steps were optimized with respect to the quantities of substrate and enzyme, and reaction time. There were particu...