Antisense wnt-5a mimics wnt-1-mediated C57MG mammary epithelial cell transformation
The disruption of the normal expression of wnt-5a in cell lines and in tumors is becoming increasingly recognized as important in cell transformation and tumorigenesis. For example, in endometrial cancer wnt-5a is downregulated compared to normal tissue. Our laboratory has recently found that the ec...
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Published in | Experimental cell research Vol. 241; no. 1; pp. 134 - 141 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
25.05.1998
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Subjects | |
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Abstract | The disruption of the normal expression of wnt-5a in cell lines and in tumors is becoming increasingly recognized as important in cell transformation and tumorigenesis. For example, in endometrial cancer wnt-5a is downregulated compared to normal tissue. Our laboratory has recently found that the ectopic expression of wnt-5a in human RCC23 renal carcinoma cells missing wnt-5a gene expression suppresses in vitro cell growth and telomerase enzyme activity. Furthermore, ectopic wnt-5a in MC-T16 uroepithelial cancer cells missing the region of chromosome 3p where wnt-5a has been mapped reverts uroepithelial cell tumorigenesis in athymic nude mice. These studies were based upon the previous finding that wnt-1 and wnt-2 transform C57MG mammary epithelial cells by downregulating the endogenous expression of wnt-5a. We now report that transfecting C57MG cells with a mammalian expression vector carrying antisense wnt-5a results in a cell phenotype that mimics cell transformation by ectopic wnt-1 or wnt-2. Correspondingly, wnt-1-transformed cells are partially reverted in the presence of ectopic wnt-5a. We conclude from this that wnt-5a is an important regulator of cell growth and differentiation and its loss of expression leads to cell transformation. |
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AbstractList | The disruption of the normal expression of wnt-5a in cell lines and in tumors is becoming increasingly recognized as important in cell transformation and tumorigenesis. For example, in endometrial cancer wnt-5a is downregulated compared to normal tissue. Our laboratory has recently found that the ectopic expression of wnt-5a in human RCC23 renal carcinoma cells missing wnt-5a gene expression suppresses in vitro cell growth and telomerase enzyme activity. Furthermore, ectopic wnt-5a in MC-T16 uroepithelial cancer cells missing the region of chromosome 3p where wnt-5a has been mapped reverts uroepithelial cell tumorigenesis in athymic nude mice. These studies were based upon the previous finding that wnt-1 and wnt-2 transform C57MG mammary epithelial cells by downregulating the endogenous expression of wnt-5a. We now report that transfecting C57MG cells with a mammalian expression vector carrying antisense wnt-5a results in a cell phenotype that mimics cell transformation by ectopic wnt-1 or wnt-2. Correspondingly, wnt-1-transformed cells are partially reverted in the presence of ectopic wnt-5a. We conclude from this that wnt-5a is an important regulator of cell growth and differentiation and its loss of expression leads to cell transformation. |
Author | Gibo, D M Olson, D J |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/9633521$$D View this record in MEDLINE/PubMed |
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SubjectTerms | Animals Cell Division - genetics Cell Division - physiology Cell Line DNA, Antisense - genetics Epithelial Cells - cytology Epithelial Cells - metabolism Female Gene Expression - genetics Mammary Glands, Animal - cytology Mammary Glands, Animal - metabolism Mice Proto-Oncogene Proteins - genetics RNA - genetics Thymidine - metabolism Transformation, Genetic - genetics Tritium Wnt Proteins Wnt-5a Protein Wnt1 Protein Zebrafish Proteins |
Title | Antisense wnt-5a mimics wnt-1-mediated C57MG mammary epithelial cell transformation |
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