Phylogeny of lymphocyte heterogeneity: the cellular requirements for the mixed leucocyte reaction with channel catfish

• Published in: Immunology Vol. 59; no. 1; pp. 123 - 128
• Main Authors: MILLER, N. W, DEUTER, A, CLEM, L. W
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell 01.09.1986
• Subjects: Animals; Antigen-Presenting Cells - immunology; B-Lymphocytes - immunology; Biological and medical sciences; Fishes - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; General aspects. Ontogeny. Phylogeny; Hot Temperature; Immunobiology; In Vitro Techniques; Leukocyte Count; Lymphocyte Culture Test, Mixed; Lymphocytes - immunology; Lymphocytes - radiation effects; Mitosis - radiation effects; Monocytes - immunology; Phylogeny; T-Lymphocytes - immunology; Heterogeneity; Primary response; Vertebrata; Immunosuppression; Mixed lymphocyte reaction; Monocyte; Pisces; T-Lymphocyte; Accessory cell; Phylogeny; Ictalurus punctatus; Lymphocyte

Vigorous mixed leucocyte reactions (MLR) were obtained using channel catfish peripheral blood leucocytes (PBL) when equal numbers of responder and stimulator cells (5 X 10(5) cells each) were cocultured. The use of 2000 rads of X-irradiation was sufficient to block subsequent proliferative responses...