An extended primer set for PCR amplification of murine kappa variable regions

The design and successful usage of an extended primer set for the PCR amplification of murine variable kappa light chain sequences from mouse hybridomas are described. Since some of these primer pairs also amplify the endogenous SP2/0 aberrant light chain sequence, strategies to distinguish the irre...

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Bibliographic Details
Published inBioTechniques Vol. 15; no. 2; p. 286
Main Authors Leung, S O, Dion, A S, Pellegrini, M C, Goldenberg, D M, Hansen, H J
Format Journal Article
LanguageEnglish
Published England 01.08.1993
Subjects
Animals
Base Sequence
Hybridomas - immunology
Immunoglobulin kappa-Chains - genetics
Immunoglobulin Variable Region - genetics
Mice
Molecular Sequence Data
Oligodeoxyribonucleotides
Polymerase Chain Reaction
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Summary:The design and successful usage of an extended primer set for the PCR amplification of murine variable kappa light chain sequences from mouse hybridomas are described. Since some of these primer pairs also amplify the endogenous SP2/0 aberrant light chain sequence, strategies to distinguish the irrelevant SP2/0 from the sequence of interest are also provided.
ISSN:0736-6205
1940-9818