Direct evidence for phorbol ester-stimulated accumulation of diacylglycerol derived from phosphatidylcholine

• Published in: Carcinogenesis (New York) Vol. 8; no. 12; p. 1943
• Main Authors: Glatz, J A, Muir, J G, Murray, A W
• Format: Journal Article
• Language: English
• Published: England 01.12.1987
• Subjects: Cell Membrane - metabolism; Chromatography, Thin Layer; Diglycerides - metabolism; Glycerides - metabolism; HeLa Cells; Humans; Phorbol Esters - pharmacology; Phosphatidylcholines - metabolism; Tetradecanoylphorbol Acetate - pharmacology

A fluorescent analog of phosphatidylcholine, 1-acyl-2-[N-(4-nitrobenzo-2-oxa-1,3-diazole)aminocaproyl]phosphatidyl choline (NBD-PC), was inserted into the plasma membrane of HeLa cells. Treatment of NBD-PC-labelled cells with the tumour promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (T...