Direct evidence for phorbol ester-stimulated accumulation of diacylglycerol derived from phosphatidylcholine

A fluorescent analog of phosphatidylcholine, 1-acyl-2-[N-(4-nitrobenzo-2-oxa-1,3-diazole)aminocaproyl]phosphatidyl choline (NBD-PC), was inserted into the plasma membrane of HeLa cells. Treatment of NBD-PC-labelled cells with the tumour promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (T...

Full description

Saved in:
Bibliographic Details
Published inCarcinogenesis (New York) Vol. 8; no. 12; p. 1943
Main Authors Glatz, J A, Muir, J G, Murray, A W
Format Journal Article
LanguageEnglish
Published England 01.12.1987
Subjects
Cell Membrane - metabolism
Chromatography, Thin Layer
Diglycerides - metabolism
Glycerides - metabolism
HeLa Cells
Humans
Phorbol Esters - pharmacology
Phosphatidylcholines - metabolism
Tetradecanoylphorbol Acetate - pharmacology
Online AccessGet more information

Cover

More Information
Summary:A fluorescent analog of phosphatidylcholine, 1-acyl-2-[N-(4-nitrobenzo-2-oxa-1,3-diazole)aminocaproyl]phosphatidyl choline (NBD-PC), was inserted into the plasma membrane of HeLa cells. Treatment of NBD-PC-labelled cells with the tumour promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), resulted in the production of fluorescent diacylglycerol, 1-acyl-2-[N-(4-nitrobenzo-2-oxa-1,3-diazole)-aminocaproyl]diacylglycerol (NBD-DG), suggesting that phorbol ester activates a PC-specific phospholipase C in these cells. Furthermore pretreatment of cells with phorbol-12,13-dibutyrate for 24 h, a process which depletes cellular protein kinase C activity, inhibited the ability of TPA to induce this effect. These results suggest that TPA stimulation of a phospholipase C specific for PC in HeLa cells is mediated via protein kinase C.
ISSN:0143-3334
DOI:10.1093/carcin/8.12.1943