Side Effect of Tris on the Interaction of Amyloid β-peptide with Cu(2+): Evidence for Tris-Aβ-Cu (2+) Ternary Complex Formation

• Published in: Applied biochemistry and biotechnology Vol. 176; no. 1; pp. 56 - 65
• Main Authors: Bin, Yannan, Jiang, Zhongxiu, Xiang, Juan
• Format: Journal Article
• Language: English
• Published: United States 01.05.2015
• Subjects: Amyloid beta-Peptides - chemistry; Copper - chemistry; Humans; Tromethamine - chemistry

The interaction of amyloid β-peptide (Aβ) with Cu(2+) is crucial to the development of neurotoxicity in Alzheimer's disease (AD). Many recent studies show a variation on the dissociation constant of Aβ-Cu(2+) under different solvent conditions. Among various buffers, the Tris(hydroxymethyl)amin...