Effect of PD-1 deficiency on atherogenic immune responses

• Published in: Zhong hua yi xue za zhi Vol. 94; no. 30; p. 2377
• Main Authors: Zhuge, Xin, Sun, Ning, Wang, Lili, Xiao, Guanghui
• Format: Journal Article
• Language: Chinese
• Published: China 13.08.2014
• Subjects: Animals; Aorta; Apolipoproteins E; Cytokines; Dendritic Cells; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Macrophages; Mice; Programmed Cell Death 1 Receptor - deficiency; Programmed Cell Death 1 Receptor - metabolism; Spleen; T-Lymphocyte Subsets; Tumor Necrosis Factor-alpha
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2014.30.016

To explore the functional changes of T lymphocyte from PD-1⁻/⁻ mice and the effects on atherogenic immune responses. PD-1⁻/⁻ mice were mated with ApoE⁻/⁻ mice to obtain PD-1⁻/⁻ApoE⁻/⁻mice. PD-1⁻/⁻ApoE⁻/⁻ mice were used as control. Both groups had a high-lipid diet for 12 weeks. Then the samples of aortic sinuses were harvested for oil-red and immunohistochemical stains. Samples of spleen and sera were obtained. Numbers of lymphocytes in spleen were evaluated by flow cytometry (FACS). And the cells of proliferation were also measured. The intracellular and serum cytokines were detected by enzyme-linked immunosorbent assay (ELISA). After a 12-week high-lipid diet, aortic root revealed more lesions in PD-1⁻/⁻ApoE⁻/⁻ mice than in PD-1⁺/⁺ ApoE⁻/⁻ controls ((0.51 ± 0.08) vs (0.29 ± 0.06) mm², t = 2.36, P < 0.01)). There was a significant increase in CD3 T cells in the lesions of PD-1⁻/⁻ApoE⁻/⁻ mice ((154.88 ± 36.64)/mm² vs (59.38 ± 25.28)/mm², t = 2.14, P < 0.01). In PD-1⁻/⁻ApoE⁻/⁻ mice, the numbers of total cells