Expression trend of selected ribosomal protein genes in nasopharyngeal carcinoma

• Published in: The Malaysian journal of medical sciences Vol. 19; no. 4; pp. 23 - 30
• Main Authors: Ma, Xiang-Ru, Sim, Edmund Ui-Hang, Ling, Teck-Yee, Tiong, Thung-Sing, Subramaniam, Selva Kumar, Khoo, Alan Soo-Beng
• Format: Journal Article
• Language: English
• Published: Malaysia Penerbit Universiti Sains Malaysia 01.10.2012
• Subjects: Original; NPC; RPS26; RPS27; RPL32; transcript expression; RP
• ISSN: 1394-195X; 2180-4303

Ribosomal proteins are traditionally associated with protein biosynthesis until recent studies that implicated their extraribosomal functions in human diseases and cancers. Our previous studies using GeneFishing™ DEG method and microarray revealed underexpression of three ribosomal protein genes, RPS26, RPS27, and RPL32 in cancer of the nasopharynx. Herein, we investigated the expression pattern and nucleotide sequence integrity of these genes in nasopharyngeal carcinoma to further delineate their involvement in tumourigenesis. The relationship of expression level with clinicopathologic factors was also statistically studied. Quantitative Polymerase Chain Reaction was performed on nasopharyngeal carcinoma and their paired normal tissues. Expression and sequence of these three genes were analysed. All three ribosomal protein genes showed no significant difference in transcript expressions and no association could be established with clinicopathologic factors studied. No nucleotide aberrancy was detected in the coding regions of these genes. There is no early evidence to substantiate possible involvement of RPS26, RPS27, and RPL32 genes in NPC tumourigenesis.