Retinoic acid can change normal differentiation of rat egg-cylinders cultured in vitro
The modified organ culture of rat egg cylinders provides favorable conditions for 2 weeks for the differentiation of main tissue types. To study the effect of retinoids on early rodent differentiation, retinoic acid (RA) was added in various concentrations to serum-supplemented or serum-free medium....
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Published in | The International journal of developmental biology Vol. 35; no. 3; pp. 197 - 202 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Bilbao
University of the Basque Country Press
01.09.1991
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Subjects | |
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Abstract | The modified organ culture of rat egg cylinders provides favorable conditions for 2 weeks for the differentiation of main tissue types. To study the effect of retinoids on early rodent differentiation, retinoic acid (RA) was added in various concentrations to serum-supplemented or serum-free medium. Explant survival decreased when RA was added to serum-free medium. Although the cartilage was well differentiated even in cultures deprived of serum, RA inhibited chondrogenesis in all cultures without or with serum. The frequency of columnar epithelium was higher and its folds more often present when RA was added to the medium. Keratinization of squamous epithelium depended on the RA concentration added to the medium, and was almost absent when the concentration was high. Other tissues often present in serum-supplemented medium (such as neuroblasts and myotubes) were not affected by RA, a result that differs from those obtained in other experimental systems. |
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AbstractList | The modified organ culture of rat egg cylinders provides favorable conditions for 2 weeks for the differentiation of main tissue types. To study the effect of retinoids on early rodent differentiation, retinoic acid (RA) was added in various concentrations to serum-supplemented or serum-free medium. Explant survival decreased when RA was added to serum-free medium. Although the cartilage was well differentiated even in cultures deprived of serum, RA inhibited chondrogenesis in all cultures without or with serum. The frequency of columnar epithelium was higher and its folds more often present when RA was added to the medium. Keratinization of squamous epithelium depended on the RA concentration added to the medium, and was almost absent when the concentration was high. Other tissues often present in serum-supplemented medium (such as neuroblasts and myotubes) were not affected by RA, a result that differs from those obtained in other experimental systems. The modified organ culture of rat egg cylinders provides favorable conditions for 2 weeks for the differentiation of main tissue types. To study the effect of retinoids on early rodent differentiation, retinoic acid (RA) was added in various concentrations to serum-supplemented or serum-free medium. The frequency of columnar epithelium was higher and its folds more often present when RA was added to the medium. |
Author | VLAHOVIC, M SKREB, N CRNEK, V |
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Keywords | Embryonic development Vertebrata Embryo Mammalia Rat Morphology Rodentia Organotypic culture Differentiation In vitro Retinoic acid |
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SubjectTerms | Animals Biological and medical sciences Cell Differentiation - drug effects Culture Media Culture Media, Serum-Free differentiation effects on egg-cylinders Embryo, Mammalian - cytology Embryo, Mammalian - drug effects Embryology: invertebrates and vertebrates. Teratology epithelium Epithelium - drug effects Experimental organogenesis Fundamental and applied biological sciences. Psychology Organ Culture Techniques Organogenesis. Physiological fonctions Rats Rats, Inbred F344 retinoic acid Tretinoin - pharmacology |
Title | Retinoic acid can change normal differentiation of rat egg-cylinders cultured in vitro |
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