Interpretation of 'Omics dynamics in a single subject using local estimates of dispersion between two transcriptomes

• Published in: AMIA ... Annual Symposium proceedings Vol. 2019; pp. 582 - 591
• Main Authors: Li, Qike, Zaim, Samir Rachid, Aberasturi, Dillon, Berghout, Joanne, Li, Haiquan, Vitali, Francesca, Kenost, Colleen, Zhang, Helen Hao, Lussier, Yves A
• Format: Journal Article
• Language: English
• Published: United States American Medical Informatics Association 2019
• Subjects: differentially expressed genes; N-of-1; RNA-Seq; single-subject; iDEG; mRNA expression

Calculating Differentially Expressed Genes (DEGs) from RNA-sequencing requires replicates to estimate gene-wise variability, a requirement that is at times financially or physiologically infeasible in clinics. By imposing restrictive transcriptome-wide assumptions limiting inferential opportunities...