Distribution of fibroblast surface antigen: association with fibrillar structures of normal cells and loss upon viral transformation

• Published in: The Journal of experimental medicine Vol. 140; no. 6; pp. 1522 - 1533
• Main Authors: Wartiovaara, J, Linder, E, Ruoslahti, E, Vaheri, A
• Format: Journal Article
• Language: English
• Published: United States The Rockefeller University Press 01.12.1974
• Subjects: Animals; Antigens - analysis; Avian Sarcoma Viruses; Cell Membrane - drug effects; Cell Membrane - immunology; Cell Transformation, Neoplastic; Cells, Cultured; Chick Embryo; Fibroblasts - immunology; Fibroblasts - ultrastructure; Fluorescent Antibody Technique; Microscopy, Electron, Scanning; Phenotype; Temperature; Trypsin - pharmacology

The localization of a cell type-specific, soluble fibroblast surface antigen (SFA) was studied by immunofluorescence and by scanning electron microscopy of the same cells. The antigen had an uneven distribution forming streaks on chick embryo fibroblasts. It was localized to membrane processes and r...