Channel gating in the absence of agonist by a homo-oligomeric molluscan GABA receptor expressed in Xenopus oocytes from a cloned cDNA

• Published in: Invertebrate neuroscience Vol. 1; no. 3; p. 267
• Main Authors: Bhandal, N S, Ramsey, R L, Harvey, R J, Darlison, M G, Usherwood, P N
• Format: Journal Article
• Language: English
• Published: Germany 01.12.1995
• Subjects: Animals; Cattle; Cloning, Molecular; DNA, Complementary - genetics; Female; gamma-Aminobutyric Acid - pharmacology; Ion Channel Gating - drug effects; Ion Channel Gating - physiology; Ion Channels - drug effects; Ion Channels - physiology; Lymnaea; Mollusca - metabolism; Oocytes - metabolism; Patch-Clamp Techniques; Receptors, GABA - chemistry; Receptors, GABA - metabolism; Virulence Factors, Bordetella - pharmacology; Xenopus laevis - metabolism

We have previously described the isolation of a complementary DNA (cDNA) from the freshwater mollusc Lymnaea stagnalis encoding a polypeptide that exhibits approximately 50% identity to the beta-subunits of vertebrate gamma-aminobutyric acid (GABA) type A (GABAA) receptor. When expressed in Xenopus...