Hyperpolarisation of cultured human chondrocytes following cyclical pressure-induced strain: evidence of a role for alpha 5 beta 1 integrin as a chondrocyte mechanoreceptor
Mechanical stimuli influence chondrocyte metabolism, inducing changes in intracellular cyclic adenosine monophosphate and proteoglycan production. We have previously demonstrated that primary monolayer cultures of human chondrocytes have an electrophysiological response after intermittent pressure-i...
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Published in | Journal of orthopaedic research Vol. 15; no. 5; pp. 742 - 744 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
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United States
01.09.1997
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Abstract | Mechanical stimuli influence chondrocyte metabolism, inducing changes in intracellular cyclic adenosine monophosphate and proteoglycan production. We have previously demonstrated that primary monolayer cultures of human chondrocytes have an electrophysiological response after intermittent pressure-induced strain characterised by a membrane hyperpolarisation of approximately 40%. The mechanisms responsible for these changes are not fully understood but potentially involve signalling molecules such as integrins that link extracellular matrix with cytoplasmic components. The results reported in this paper demonstrate that the transduction pathways involved in the hyperpolarisation response of human articular chondrocytes in vitro after cyclical pressure-induced strain involve alpha 5 beta 1 integrin. We have demonstrated, using pharmacological inhibitors of a variety of intracellular signalling pathways, that the actin cytoskeleton, the phospholipase C calmodulin pathway, and both tyrosine protein kinase and protein kinase C activities are important in the transduction of the electrophysiological response. These results suggest that alpha 5 beta 1 is an important chondrocyte mechanoreceptor and a potential regulator of chondrocyte function. |
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AbstractList | Mechanical stimuli influence chondrocyte metabolism, inducing changes in intracellular cyclic adenosine monophosphate and proteoglycan production. We have previously demonstrated that primary monolayer cultures of human chondrocytes have an electrophysiological response after intermittent pressure-induced strain characterised by a membrane hyperpolarisation of approximately 40%. The mechanisms responsible for these changes are not fully understood but potentially involve signalling molecules such as integrins that link extracellular matrix with cytoplasmic components. The results reported in this paper demonstrate that the transduction pathways involved in the hyperpolarisation response of human articular chondrocytes in vitro after cyclical pressure-induced strain involve alpha 5 beta 1 integrin. We have demonstrated, using pharmacological inhibitors of a variety of intracellular signalling pathways, that the actin cytoskeleton, the phospholipase C calmodulin pathway, and both tyrosine protein kinase and protein kinase C activities are important in the transduction of the electrophysiological response. These results suggest that alpha 5 beta 1 is an important chondrocyte mechanoreceptor and a potential regulator of chondrocyte function. |
Author | Salter, D M Bavington, C Dunne, E Jobanputra, P Godolphin, J L Nishida, K Walmsley, S Wright, M O Nuki, G |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/9420605$$D View this record in MEDLINE/PubMed |
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SubjectTerms | Actins - metabolism Biological membranes Biomechanics Cartilage, Articular - cytology Cartilage, Articular - drug effects Cartilage, Articular - metabolism Cells, Cultured Chondrocytes - drug effects Chondrocytes - metabolism Electrophysiology Enzyme inhibition Enzyme kinetics Humans Integrin beta1 - pharmacology Mechanical properties Mechanoreceptors - drug effects Mechanoreceptors - physiology Membrane Potentials - drug effects Metabolism Oligopeptides - pharmacology Protein Kinase C - metabolism Protein-Tyrosine Kinases - metabolism Proteins Receptors, Fibronectin - drug effects Receptors, Fibronectin - physiology Signal Transduction - drug effects Space life sciences Stress, Mechanical Type C Phospholipases - metabolism |
Title | Hyperpolarisation of cultured human chondrocytes following cyclical pressure-induced strain: evidence of a role for alpha 5 beta 1 integrin as a chondrocyte mechanoreceptor |
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