A Method to Collect Cerebrospinal Fluid from Mouse Cisterna Magna to Determine Extracellular Tau Levels

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2754; p. 343
• Main Authors: Ishida, Kazuhisa, Yamada, Kaoru
• Format: Journal Article
• Language: English
• Published: United States 2024
• Subjects: Alzheimer’s disease; Tau protein; ISF; CSF; Extracellular tau
• ISSN: 1940-6029
• DOI: 10.1007/978-1-0716-3629-9_18

Despite being a cytoplasmic protein abundant in neurons, tau is detectable in various extracellular fluids. In addition to being passively released from dying/degenerating neurons, tau is also actively released from living neurons in a neuronal activity-dependent mechanism. In vivo, tau released fro...