Triple-Color FluoroSpot Analysis of Polyfunctional Antigen-Specific T Cells by Quantification of Spot-Forming Units and Relative Spot Volumes

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2768; p. 297
• Main Authors: Ahlborg, Niklas, Smedman, Christian, Makower, Bartek
• Format: Journal Article
• Language: English
• Published: United States 2024
• Subjects: Antigens; Coloring Agents; Cytokines - analysis; Enzyme-Linked Immunospot Assay; T-Lymphocytes - chemistry; FluoroSpot; Polyfunctional T cells; ELISpot; Cytokine

Switching from ELISpot to FluoroSpot enables the analysis of spot-forming units representing cells producing different cytokines as well as the frequencies of spots derived from cells co-secreting multiple cytokines. Due to the fluorescent read-out signal, sophisticated reader instruments can also m...