Analysis of Suppressor of Cytokine Signalling (SOCS) gene expression by real-time quantitative PCR

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 967; p. 235
• Main Authors: Kolesnik, Tatiana B, Nicholson, Sandra E
• Format: Journal Article
• Language: English
• Published: United States 2013
• Subjects: Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; DNA Primers - genetics; DNA, Complementary - biosynthesis; Gene Expression Regulation; Humans; Interleukin-4 - pharmacology; Macrophages - metabolism; Real-Time Polymerase Chain Reaction - methods; Real-Time Polymerase Chain Reaction - standards; Reference Standards; Suppressor of Cytokine Signaling Proteins - genetics

The Suppressor of Cytokine Signalling (SOCS) proteins are a family of negative regulators characterized by a central SH2 domain and C-terminal SOCS box motif. Cytokine Inducible SH2-containing protein (CIS), SOCS1, 2 and 3 are rapidly upregulated in response to cytokine stimulation and act to inhibi...