Genetic Modification of Tumor-Infiltrating Lymphocytes, Peripheral T Cells, and T-Cell Model Cell Lines

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2748; p. 167
• Main Authors: Weinstein-Marom, Hadas, Blokon-Kogan, Dayana, Levi-Mann, Maya, Katzman, Chaja, Shalev, Shira, Zaitsev, Masha, Besser, Michal J, Shapira-Frommer, Ronnie, Gross, Gideon, Itzhaki, Orit, Nissim, Lior
• Format: Journal Article
• Language: English
• Published: United States 2024
• Subjects: Chimeric antigen receptors; Retroviral transduction; Preparation of T cells; T cell activation; mRNA electroporation; Rapid expansion protocol; Adoptive cell therapy; Tumor-infiltrating lymphocyte; CD8 T cells
• ISSN: 1940-6029
• DOI: 10.1007/978-1-0716-3593-3_13

Genetic modification of tumor-infiltrating lymphocytes (TILs) or circulating T cells has become an important avenue in cancer therapy. Here we describe a comprehensive method for establishing and expanding TIL cultures and genetically modifying them with a gene of interest (GOI) via retroviral transduction or mRNA transfection. The method includes all the important steps starting with TIL extraction from tumors through to the maintenance of the genetically modified TILs. The protocol includes instructions for retroviral transduction and mRNA transfection of circulating T cells or T-cell lines. The GOIs most commonly introduced into the target cells are chimeric antigen receptors (CARs); genetic adjuvants, such as membrane-bound interleukins; and antitumor T-cell receptors (TCRs).