Misexpression approaches for the manipulation of flower development

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1110; p. 383
• Main Authors: Xu, Yifeng, Gan, Eng-Seng, Ito, Toshiro
• Format: Journal Article
• Language: English
• Published: United States 2014
• Subjects: Agrobacterium - genetics; Arabidopsis - genetics; Cloning, Molecular; Dexamethasone - pharmacology; Flowers - drug effects; Flowers - genetics; Flowers - growth & development; Gene Expression - drug effects; Genes, Plant - genetics; Genetic Engineering - methods; Organ Specificity; Phenotype; Promoter Regions, Genetic - genetics; Protein Biosynthesis - drug effects; Transcription, Genetic - drug effects; Transformation, Genetic

The generation of dominant gain-of-function mutants through activation tagging is a forward genetic approach that complements the screening of loss-of-function mutants and that has been successfully applied to studying the mechanisms of flower development. In addition, the functions of genes of inte...