Misexpression Approaches for the Manipulation of Flower Development

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2686; p. 429
• Main Authors: Xu, Yifeng, Gan, Eng-Seng, Ito, Toshiro
• Format: Journal Article
• Language: English
• Published: United States 2023
• Subjects: Promoter; Activation tagging; Misexpression; Overexpression; CRISPR-Cas9; Glucocorticoid receptor

The generation of dominant gain-of-function mutants through activation tagging is a forward genetic approach that can be applied to study the mechanisms of flower development, complementing the screening of loss-of-function mutants. In addition, the functions of genes of interest can be further anal...