Characterizing Candida glabrata Pdr1, a Hyperactive Transcription Factor Involved in Azole Resistance

This chapter illustrates how to prepare isogenic strains carrying gain-of-function forms of transcription factor Pdr1 in the human pathogen Candida glabrata. Simple steps are described that lead from a characterized plasmid-borne PDR1-GOF allele to its integration into the yeast genome in a markerle...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2658; p. 169
Main Authors Simonicova, Lucia, Moye-Rowley, W Scott
Format Journal Article
LanguageEnglish
Published United States 2023
Subjects
Animals
Antifungal Agents - pharmacology
Azoles - pharmacology
Candida glabrata - genetics
DNA-Binding Proteins - metabolism
Drug Resistance, Fungal - genetics
Fluconazole - pharmacology
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Expression Regulation, Fungal
Humans
Microbial Sensitivity Tests
Transcription Factors - genetics
Transcription Factors - metabolism
Candida glabrata
Azole resistance
Marker-less integration of PDR1-GOF into the genome
Isogenic strains
Transcription factor Pdr1
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Summary:This chapter illustrates how to prepare isogenic strains carrying gain-of-function forms of transcription factor Pdr1 in the human pathogen Candida glabrata. Simple steps are described that lead from a characterized plasmid-borne PDR1-GOF allele to its integration into the yeast genome in a markerless manner. Pdr1-GOF strains constructed by this approach are suitable for virulence studies in an animal host.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3155-3_11