Considerations for Glycoprotein Production

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2762; p. 329
• Main Author: Clarke, Elizabeth C
• Format: Journal Article
• Language: English
• Published: United States 2024
• Subjects: Animals; CHO Cells; Cricetinae; Cricetulus; Glycoproteins - chemistry; Glycosylation; Humans; Recombinant Proteins - metabolism; Expression system; Escherichia coli; Glycoprotein; Human endothelial kidney; O-linked glycosylation; Glycans; Glycosylation; Complex glycans; Pichia pastoris; Baculovirus; Chinese hamster ovary cells; Recombinant protein production; N-linked glycosylation

This chapter is intended to provide insights for researchers aiming to choose an appropriate expression system for the production of recombinant glycoproteins. Producing glycoproteins is complex, as glycosylation patterns are determined by the availability and abundance of specific enzymes rather th...