Complementation Assays for Co-chaperone Function

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2693; p. 105
• Main Authors: Edkins, Adrienne L, Blatch, Gregory L
• Format: Journal Article
• Language: English
• Published: United States 2023
• Subjects: Heat shock proteins; J domain proteins; DnaJ; Molecular chaperones; Protein folding; Hsp40

The development of mutant microorganisms lacking J domain proteins (JDPs; formerly called Hsp40s) has enabled the development of complementation assays for testing the co-chaperone function of JDPs. In these assays, an exogenously expressed novel JDP is tested for its ability to functionally substit...