Characterization of the interaction between the prostaglandin D2 DP1 receptor and the intracellular L-prostaglandin D synthase
Identification of G protein-coupled receptor (GPCR)-interacting proteins is an intense subject of current research. However, confirmation and characterization of identified interactions can be difficult with GPCRs, especially at the endogenous level. Here, we describe how we characterized the intera...
Saved in:
Published in | Methods in molecular biology (Clifton, N.J.) Vol. 1234; p. 53 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
2015
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Identification of G protein-coupled receptor (GPCR)-interacting proteins is an intense subject of current research. However, confirmation and characterization of identified interactions can be difficult with GPCRs, especially at the endogenous level. Here, we describe how we characterized the interaction between the prostaglandin D2 DP1 receptor and the intracellular L-type prostaglandin D synthase by in vitro pull-down assays using purified recombinant GST- and His-tagged proteins, by co-immunoprecipitation of overexpressed Flag- and HA-tagged proteins, and by co-immunoprecipitation of endogenous proteins. |
---|---|
AbstractList | Identification of G protein-coupled receptor (GPCR)-interacting proteins is an intense subject of current research. However, confirmation and characterization of identified interactions can be difficult with GPCRs, especially at the endogenous level. Here, we describe how we characterized the interaction between the prostaglandin D2 DP1 receptor and the intracellular L-type prostaglandin D synthase by in vitro pull-down assays using purified recombinant GST- and His-tagged proteins, by co-immunoprecipitation of overexpressed Flag- and HA-tagged proteins, and by co-immunoprecipitation of endogenous proteins. |
Author | Parent, Jean-Luc Binda, Chantal |
Author_xml | – sequence: 1 givenname: Chantal surname: Binda fullname: Binda, Chantal organization: Department of Medicine, Université de Sherbrooke, Sherbrooke, QC, Canada – sequence: 2 givenname: Jean-Luc surname: Parent fullname: Parent, Jean-Luc |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25304348$$D View this record in MEDLINE/PubMed |
BookMark | eNpdkMtKxDAUhoMozkWfQJC8QDQ5uTRZSsdRYUAXuh4y7amtdNKSZpBx4bNbL7NxdeD7-X74z4wchy4gIReCXwnOs2uXWSaYctIxkWnNzNockalwijPDwU3IbBjeOFeZBHVKJqAlV1LZKfnMax99kTA2Hz41XaBdRVONtAkjG4NvtMH0jhh-eB-7IfnX1oeyCXQBdPEkaMQC-9RFOtKDPcrYtrvWR7pi_yw67EOq_YBn5KTy7YDnf3dOXpa3z_k9Wz3ePeQ3oyiMNUwUXBUAlR8XgVGlk4CuLLguuXLCZ1A5XWlQG4nWWhQ2s6YC0OCl09p5mJPL395-t9liue5js_Vxvz48Ar4AAxlhWg |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.1007/978-1-4939-1755-6_6 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Biology |
EISSN | 1940-6029 |
ExternalDocumentID | 25304348 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GrantInformation_xml | – fundername: Canadian Institutes of Health Research |
GroupedDBID | --- 29M 53G ACGFS ALMA_UNASSIGNED_HOLDINGS CGR CUY CVF ECM EIF F5P NPM P2P RSU SPO UDS WH7 ZGI |
ID | FETCH-LOGICAL-p1686-1c04c22fa194264d932e9dc05d0491a72f95f524b3e888e18786f2252a39559a2 |
IngestDate | Thu May 23 23:20:11 EDT 2024 |
IsPeerReviewed | false |
IsScholarly | true |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-p1686-1c04c22fa194264d932e9dc05d0491a72f95f524b3e888e18786f2252a39559a2 |
PMID | 25304348 |
ParticipantIDs | pubmed_primary_25304348 |
PublicationCentury | 2000 |
PublicationDate | 2015-00-00 |
PublicationDateYYYYMMDD | 2015-01-01 |
PublicationDate_xml | – year: 2015 text: 2015-00-00 |
PublicationDecade | 2010 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Methods in molecular biology (Clifton, N.J.) |
PublicationTitleAlternate | Methods Mol Biol |
PublicationYear | 2015 |
SSID | ssj0047324 |
Score | 2.1435745 |
Snippet | Identification of G protein-coupled receptor (GPCR)-interacting proteins is an intense subject of current research. However, confirmation and characterization... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 53 |
SubjectTerms | Cell Culture Techniques Cell Line Humans Immunoprecipitation - methods Intracellular Space - metabolism Intramolecular Oxidoreductases - genetics Intramolecular Oxidoreductases - metabolism Lipocalins - genetics Lipocalins - metabolism Protein Binding Receptors, Immunologic - genetics Receptors, Immunologic - metabolism Receptors, Prostaglandin - genetics Receptors, Prostaglandin - metabolism Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism |
Title | Characterization of the interaction between the prostaglandin D2 DP1 receptor and the intracellular L-prostaglandin D synthase |
URI | https://www.ncbi.nlm.nih.gov/pubmed/25304348 |
Volume | 1234 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV07T8MwELYKCIkF8X4jD2yVUeM8PUILQqggBpDYqsR1BANphcIAAyt_m8923IbwELBEld1Ylb_r-e589x0hB2KYCRkngsVw2-CgRJzBs80YThKBE08JT-o45MVldHYTnN-Gt63WWy1r6anMDuXLl3Ul_0EVY8BVV8n-AdnJohjAZ-CLJxDG81cYdydsyy8Ty08bkpoD4rFqAu4SsWxJ1AjGoOnacV-0e7zdu_LaUHlqXNZyKe91vFcH9E2Gap813tIkB-Wdu9RxzaBMI2qTW_vgGu62HcGTvSjOS1fbdX5Yiz8c66BAdfGvKzOnd1qaN8om4KQF6z_JeoDCC2sBCmWVqtAlBp1qZad1eRXEtIrTMgZ_0ufTFA54usIXDNZOyKJBVP82QBk_GIh56HcC31J3_jzbINl2UzNkJk60orzUQR97oAcxjM4JYZXlJG78Gk0pXa3QcE-MmXK9RBYr_4IeWWFZJi1VrJB523H0eZW8NkWGjnIK0GlNZGglMmb8A_i0xylEhjqRoRh1b09FhjZEhvaoE5k1cnN6ct09Y1ULDjb2oiRinuwEkvM8BYYwnYew9pUYyk44hGfppTHPRZiHPMh8lSSJ8hLsXo4jgqe-pjZM-TqZLUaF2iQ0lQLeiZ_HMg2CLA4y6Zk7ew4bOIbRukU27MYNxpZnZeC2dPvbmR2yMJW4XTKX44-t9mAlltm-wfAdlRdlLQ |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Characterization+of+the+interaction+between+the+prostaglandin+D2+DP1+receptor+and+the+intracellular+L-prostaglandin+D+synthase&rft.jtitle=Methods+in+molecular+biology+%28Clifton%2C+N.J.%29&rft.au=Binda%2C+Chantal&rft.au=Parent%2C+Jean-Luc&rft.date=2015-01-01&rft.eissn=1940-6029&rft.volume=1234&rft.spage=53&rft_id=info:doi/10.1007%2F978-1-4939-1755-6_6&rft_id=info%3Apmid%2F25304348&rft_id=info%3Apmid%2F25304348&rft.externalDocID=25304348 |