Preparation of mineralized tissue for light microscopy

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 915; p. 37
• Main Authors: Valentine, Gail, Piper, Kim
• Format: Journal Article
• Language: English
• Published: United States 2012
• Subjects: Animals; Bone and Bones - anatomy & histology; Calcification, Physiologic; Decalcification Technique; Humans; Immunohistochemistry; Microscopy - methods; Staining and Labeling; Tissue Fixation; Tooth - anatomy & histology

Production of stained tissue sections for examination by light microscopy is a step-wise process which begins with preservation of tissue (fixation), then dehydration and clearing of the tissue, and finally impregnation with wax (processing). Mineralized tissues such as bone and teeth are subject to...