Inhibition of cPLA2 and sPLA2 Activities in Primary Cultures of Rat Cortical Neurons by Centella asiatica Water Extract
Leaf extract of Centella asiatica has been used as an alternative medicine for memory improvement in the Indian Ayurvedic system of medicine for a long time. Although several studies have revealed its effect in ameliorating the cognitive impairment in rat models of Alzheimer's disease, the mole...
Saved in:
Published in | Natural product communications Vol. 7; no. 7; pp. 841 - 843 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Los Angeles, CA
SAGE Publications
01.07.2012
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Leaf extract of Centella asiatica has been used as an alternative medicine for memory improvement in the Indian Ayurvedic system of medicine for a long time. Although several studies have revealed its effect in ameliorating the cognitive impairment in rat models of Alzheimer's disease, the molecular mechanism of C. asiatica on neuroprotection still remains unexplained. In this study, we investigated the effects of C. asiatica water extract on activity of subtypes of phospholipase A2 (PLA2) in primary cultures of rat cortical neurons and quantified by HPLC a possible molecule responsible for the activity. The cPLA2 and sPLA2 activities were inhibited in vitro by asiaticoside present in the water extract of C. asiatica. This extract may be a candidate for the treatment of neurodegenerative processes because of its pharmacological activity in the brain and its low toxicity, as attested by its long popular use as a natural product. |
---|---|
AbstractList | Leaf extract of Centella asiatica has been used as an alternative medicine for memory improvement in the Indian Ayurvedic system of medicine for a long time. Although several studies have revealed its effect in ameliorating the cognitive impairment in rat models of Alzheimer's disease, the molecular mechanism of C. asiatica on neuroprotection still remains unexplained. In this study, we investigated the effects of C. asiatica water extract on activity of subtypes of phospholipase A2 (PLA2) in primary cultures of rat cortical neurons and quantified by HPLC a possible molecule responsible for the activity. The cPLA2 and sPLA2 activities were inhibited in vitro by asiaticoside present in the water extract of C. asiatica. This extract may be a candidate for the treatment of neurodegenerative processes because of its pharmacological activity in the brain and its low toxicity, as attested by its long popular use as a natural product. |
Author | Raposo, André H. Ferreira, Aline S. Gattaz, Wagner F. Polonini, Hudson C. Fedoce, Alessandra G. Raposo, Nádia R. B. Defillipo, Patrícia P. |
Author_xml | – sequence: 1 givenname: Patrícia P. surname: Defillipo fullname: Defillipo, Patrícia P. – sequence: 2 givenname: André H. surname: Raposo fullname: Raposo, André H. – sequence: 3 givenname: Alessandra G. surname: Fedoce fullname: Fedoce, Alessandra G. – sequence: 4 givenname: Aline S. surname: Ferreira fullname: Ferreira, Aline S. – sequence: 5 givenname: Hudson C. surname: Polonini fullname: Polonini, Hudson C. – sequence: 6 givenname: Wagner F. surname: Gattaz fullname: Gattaz, Wagner F. – sequence: 7 givenname: Nádia R. B. surname: Raposo fullname: Raposo, Nádia R. B. |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/22908561$$D View this record in MEDLINE/PubMed |
BookMark | eNplkctOwzAQRS1UREvpD7BAXrIJtZM4dpZVVaBSBRUCwS7yK-AqdYrt8Ph7HFpWWCP5ynPmajxzCga2tRqAc4yuMKZ0isssJ5S94BQh2kd5BEaYEJKUOSWDqCOQ9MQQTLzfoHgYy1FenoBhmpaIkQKPwOfSvhlhgmktbGso16tZCrlV0P-qmQzmI2a1h8bCtTNb7r7hvGtC5-JbrHjgAc5bF4zkDbzTnWuthyIy2gbdNBxyb3ifhc88aAcXX8FxGc7Acc0bryeHewyerheP89tkdX-znM9WyQ4XRUiIkkoiiVGWISQZzwqlKdNCyLogmChalgUpmSBYMJrWQhGkkcC8zilVjLJsDC73vjvXvnfah2prvOwbs7rtfBWdc4ZIhmhELw5oJ7ZaVbv9b6u_YUVgugc8f9XVpu2cja1Hi6rfSPV_I9kPwzd8NA |
ContentType | Journal Article |
Copyright | 2012 SAGE Publications Inc. |
Copyright_xml | – notice: 2012 SAGE Publications Inc. |
DBID | CGR CUY CVF ECM EIF NPM 7X8 |
DOI | 10.1177/1934578X1200700709 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Chemistry |
EISSN | 1555-9475 |
EndPage | 843 |
ExternalDocumentID | 22908561 10.1177_1934578X1200700709 |
Genre | Journal Article |
GroupedDBID | --- 0R~ 123 31X 53G 54M AADUE AAFWJ AARIX AATBZ ABDNZ ABKRH ABQXT ABRHV ACLDX ACOFE ACROE ACSIQ ACUIR ADOGD AENEX AEWDL AEWHI AEXNY AFCOW AFKRG AFPKN AFRWT AFUIA AGNHF AIOMO AJUZI ALMA_UNASSIGNED_HOLDINGS BDDNI CFDXU DOPDO DV7 EBS EJD F5P GROUPED_DOAJ GROUPED_SAGE_PREMIER_JOURNAL_COLLECTION HZ~ J8X JCYGO O9- OK1 ROL SAFTQ SFC SFK SFT SGV SJN SPP ZPPRI ZRKOI CGR CUY CVF ECM EIF H13 NPM 7X8 |
ID | FETCH-LOGICAL-p166t-5dcdc0c103300c8a36de78ebbcf6515d7996598b51b872fbd50e0b1af477d8783 |
IEDL.DBID | AFRWT |
ISSN | 1934-578X |
IngestDate | Fri Aug 16 23:45:19 EDT 2024 Sat Sep 28 08:03:59 EDT 2024 Tue Jul 16 20:53:21 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 7 |
Keywords | Centella asiatica Primary culture Phospholipase A2 Umbelliferae Neurons |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-p166t-5dcdc0c103300c8a36de78ebbcf6515d7996598b51b872fbd50e0b1af477d8783 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 22908561 |
PQID | 1034805307 |
PQPubID | 23479 |
PageCount | 3 |
ParticipantIDs | proquest_miscellaneous_1034805307 pubmed_primary_22908561 sage_journals_10_1177_1934578X1200700709 |
PublicationCentury | 2000 |
PublicationDate | 20120700 2012-Jul 20120701 |
PublicationDateYYYYMMDD | 2012-07-01 |
PublicationDate_xml | – month: 7 year: 2012 text: 20120700 |
PublicationDecade | 2010 |
PublicationPlace | Los Angeles, CA |
PublicationPlace_xml | – name: Los Angeles, CA – name: United States |
PublicationTitle | Natural product communications |
PublicationTitleAlternate | Nat Prod Commun |
PublicationYear | 2012 |
Publisher | SAGE Publications |
Publisher_xml | – name: SAGE Publications |
References | Barbosa, Pittella, Gattaz 2008; 15 Moses, Jensen, Lue, Walker, Sun, Simonyi, Sun 2006; 3 Bezzi, Carmifnoto, Pasti, Vesce, Rossi, Lodi Rizzini, Pozzan, Volterra 1998; 391 Miyake, Yamamoto, Takebayashi, Imai, Honda 1992; 263 Stranahan, Mattson 2012; 13 Sanfeliu, Hunt, Patel 1990; 526 Dhanasekaran, Holcomb, Hitt, Tharakan, Porter, Young, Manyam 2009; 23 Mook-Jung, Shin, Yun, Huh, Koh, Park, Jew, Jung 1999; 58 Gattaz, Schmitt, Maras 1995; 16 Malaplate-Armand, Florent-Béchard, Youssef, Koziel, Sponne, Kriem, Leininger-Muller, Olivier, Oster, Pillot 2006; 23 Schaeffer, Gattaz 2008; 198 Veerendra Kumar, Gupta 2003; 30 Bonfill, Mangas, Cusidó, Osuna, Piñol, Palazón 2006; 20 Nirmal, Praba, Velmurugan 2008; 45 Lowry, Rowebrough, Farr, Randall 1951; 193 Park, Paek, Lee, Kim, Lee, Choi, Yong, Kim 2007; 30 |
References_xml | – volume: 45 start-page: 256 year: 2008 end-page: 262 article-title: Modeling studies on phospholipase A -inhibitor complexes publication-title: Indian Journal of Biochemistry & Biophysics contributor: fullname: Velmurugan – volume: 58 start-page: 417 year: 1999 end-page: 425 article-title: Protective effects of asiaticoside derivatives against beta-amyloid neurotoxicity publication-title: Journal of Neuroscience Research contributor: fullname: Jung – volume: 30 start-page: 176 year: 2007 end-page: 179 article-title: Inhibitory effects of asiatic acid on 7,12-dimethylbenz[a]anthrax-cene and 12-O-tetradecanoylphorbol 13-acetate-induced tumor promotion in mice publication-title: Biological & Pharmaceutical Bulletin contributor: fullname: Kim – volume: 16 start-page: 1 year: 1995 end-page: 6 article-title: Increased platelet phospholipase A activity in schizophrenia publication-title: Schizophrenia Research contributor: fullname: Maras – volume: 391 start-page: 281 year: 1998 end-page: 285 article-title: Prostaglandins stimulate calcium dependent glutamate release in astrocytes publication-title: Nature contributor: fullname: Volterra – volume: 193 start-page: 265 year: 1951 end-page: 275 article-title: Protein measurement with the Folin phenol reagent publication-title: Journal of Biological Chemistry contributor: fullname: Randall – volume: 263 start-page: 1302 year: 1992 end-page: 1307 article-title: The novel natural product YM-26567-1 [(+)-trans-4-(3-dodecanoyl-2,4,6-trihydroxyphenyl)-7-hydroxy-2-(4-hydroxyphenyl)chroman]: a competitive inhibitor of group II phospholipase A2 publication-title: Journal of Pharmacology and Experimental Therapeutics contributor: fullname: Honda – volume: 30 start-page: 336 year: 2003 end-page: 342 article-title: Effect of Centella asiatica on cognition and oxidative stress in an intracerebroventricular streptozotocin model of Alzheimer's disease in rats publication-title: Clinical and Experimental Pharmacology and Physiology contributor: fullname: Gupta – volume: 23 start-page: 14 year: 2009 end-page: 19 article-title: Centella asiatica extract selectively decreases amyloid beta levels in hippocampus of Alzheimer's disease animal model publication-title: Phytotherapy Research contributor: fullname: Manyam – volume: 23 start-page: 178 year: 2006 end-page: 189 article-title: Soluble oligomers of amyloid-beta peptide induce neuronal apoptosis by activating a cPLA -dependent sphingomyelinase-ceramide pathway publication-title: Neurobiology of Disease contributor: fullname: Pillot – volume: 3 start-page: 28 year: 2006 end-page: 38 article-title: Secretory PLA2-IIA: a new inflammatory factor for Alzheimer's disease publication-title: Journal of Neuroinflammation contributor: fullname: Sun – volume: 20 start-page: 151 year: 2006 end-page: 153 article-title: Identification of triterpenoid compounds of Centella asiatica by thin-layer chromatography and mass spectrometry publication-title: Biomedical Chromatography contributor: fullname: Palazón – volume: 13 start-page: 209 year: 2012 end-page: 216 article-title: Recruiting adaptive cellular stress responses for successful brain ageing publication-title: Nature Review Neuroscience. contributor: fullname: Mattson – volume: 526 start-page: 241 year: 1990 end-page: 248 article-title: Exposure to N-methyl-D-aspartate increases release of arachidonic acid in primary cultures of rat hippocampal neurons and not in astrocytes publication-title: Brain Research contributor: fullname: Patel – volume: 198 start-page: 1 year: 2008 end-page: 27 article-title: Cholinergic and glutamatergic alterations beginning at the early stages of Alzheimer disease: participation of the phospholipase A2 enzyme publication-title: Psychopharmacology contributor: fullname: Gattaz – volume: 15 start-page: 896 year: 2008 end-page: 900 article-title: Centella asiatica water extract inhibits iPLA2 and cPLA2 activities in rat cerebellum publication-title: Phytomedicine contributor: fullname: Gattaz |
SSID | ssj0000884049 |
Score | 2.010323 |
Snippet | Leaf extract of Centella asiatica has been used as an alternative medicine for memory improvement in the Indian Ayurvedic system of medicine for a long time.... |
SourceID | proquest pubmed sage |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 841 |
SubjectTerms | Animals Enzyme Activation - drug effects Female Neurons - enzymology Phospholipases A2, Cytosolic - metabolism Phospholipases A2, Secretory - metabolism Plant Leaves - chemistry Pregnancy Rats Rats, Wistar Triterpenes - chemistry Triterpenes - pharmacology Water - chemistry |
Title | Inhibition of cPLA2 and sPLA2 Activities in Primary Cultures of Rat Cortical Neurons by Centella asiatica Water Extract |
URI | https://journals.sagepub.com/doi/full/10.1177/1934578X1200700709 https://www.ncbi.nlm.nih.gov/pubmed/22908561 https://search.proquest.com/docview/1034805307 |
Volume | 7 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1LS8NAEF6qPehFfFtfrODBy9qkSbqbk5TSoqIiorS3sE_0YFraFPXfO5NNBLEHIYeQbNhkdnbz7Ty-IeScq8ipKE5ZKoVjsbOKyY7lLFE6tdw540f6_qF7_RLfjpNxg0zrXJhKgvNLDKuCNyoXa5zdaI1uV07GNqCOGFRtHKKlDY_0alG8Z97eXZfVwCvooF68o29bY0TkF6vz21ZIswO7P5gTzd7wafT8Y5eBWRd71IydMOylzrVZ2vEyZPorKqz8UQ03yUaFMGnPq8QWadh8m6z168JuO-TjJn99U2WoFp04qh_veh0qc0Pn5VlPlwUlYAdN33L66NkoqKfehGvwxJMsaH8yK63gtGT3yOdUQRvP7ykpZmbiXToCJDujg88Cc7F2yctw8Ny_ZlX9BTYNu92CJUYbHegwiKIg0EJGXWO5sEpphwXUDU-RjVCoJFSCd5wySWADFUoXc24EF9EeWc0nuT0gVGmLzPaAnVIbp5EB3JGI1ARWi1BLZ1vkrJZiBtJAp4XM7WQxz6D3WMBKEfAW2ffizab-0zPkqhcAAFvkAuWd1cqThRWJ-d_hOvx_0yOyDuiois09JqvFbGFPAIEU6rRSmm8efdWl |
link.rule.ids | 315,786,790,21994,27886,27957,27958,44980,45368 |
linkProvider | SAGE Publications |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1LS8NAEB5qe6gX8W19ruDBSyTpbrK7eCrF0moVkRZ7C_vEXlJpI_rz3c2jIHoQcgjJTh4zs8mXycw3AFdUYisx4QEXzAbEGhmIrqFBLBU31FpdWvrxKRlOyf0snjXgtq6FqTS4uvFpVe6Kiof1enb7OnGOifOyWeSDbH7hG9AiMU5YE1q9wcvrZB1icROIlADYCwVeqi6b-fNAf4HMHwlexTtnsA1bFVhEvdK6O9Aw2S60-3WPtj34HGVvc1lkXaGFRep53OsikWm0KtZ6qugN4T6G0TxDzyWxBCpZNN02J_EictRfLIuANiqIOrIVkm5MSdUpkC-y9HvRqwOlS3T3lfuyqn2YDu4m_WFQtVII3qMkyYNYK61CFYUYh6FiAifaUGakVNb3QteUe2JBJuNIMtq1UsehCWUkLKFUM8rwATSzRWaOAEllPEm9g0HcEI61gxAx4zo0ikVKWNOBy1qLqdOG__8gMrP4WKXu7IS5SR_SDhyW6k3fy1tPPe08c1iuA9de32ntB2lU8ZH_Ntfx_4deQHs4eRyn49HTwwlsOtBTpdyeQjNffpgzByxyeV450DeHB8MJ |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1LT8MwDLZgSMAF8WY8g8SBSyFd0yU5ToOJx5gmBGK3Kk_BpZu2Ivj5OG2HhOCA1EPVJn3YTvPVsT8DnHGdeJ0wGUklfMS805FqOR6l2kjHvbeVph8G7ZtndjdKR3Wd05ALU0twdhHCqvCJyo91GN0T6y_rNcZLBB0MLW0UB0db2OQiLDEmBW3AUqf3-PL07WbBQcQqEBw6RaHXPHXmzwv9BTR_BHmV805vHdZqwEg6lYY3YMHlm7DSnddp24KP2_z1TZeRV2TsiRn2Oy2icktm5V7HlPUh8IeYvOVkWJFLkIpJE49hj0dVkO54Wjq1SUnWkc-IxjYVXaciIdEynCUvCEyn5PqzCKlV2_Dcu37q3kR1OYVoErfbRZRaYw01MU0SSo1QSds6LpzWxod66JbLQC4odBprwVte25Q6qmPlGedWcJHsQCMf524PiDYuENUjFJKOycQijEiFtNQZERvlXRNO51LMUBphDULlbvw-y_DuTODAp7wJu5V4s0n16lmgnheI55pwHuSdzW0hi2tO8t_q2v9_0xNYHl71sv7t4P4AVhH31FG3h9Aopu_uCLFFoY9r-_kCmWLEGQ |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Inhibition+of+cPLA2+and+sPLA2+Activities+in+Primary+Cultures+of+Rat+Cortical+Neurons+by+Centella+asiatica+Water+Extract&rft.jtitle=Natural+product+communications&rft.au=Defillipo%2C+Patr%C3%ADcia+P.&rft.au=Raposo%2C+Andr%C3%A9+H.&rft.au=Fedoce%2C+Alessandra+G.&rft.au=Ferreira%2C+Aline+S.&rft.date=2012-07-01&rft.pub=SAGE+Publications&rft.issn=1934-578X&rft.eissn=1555-9475&rft.volume=7&rft.issue=7&rft_id=info:doi/10.1177%2F1934578X1200700709&rft.externalDocID=10.1177_1934578X1200700709 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1934-578X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1934-578X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1934-578X&client=summon |