Microbiological study of the subgingival biofilm in HIV+/HAART patients at a specialized dental service

• Published in: Acta odontológica latinoamericana Vol. 32; no. 3; pp. 147 - 155
• Main Authors: Gliosca, Laura A, D Eramo, Luciana R, Bozza, Florencia L, Soken, Luciana, Abusamra, Lorena, Salgado, Pablo A, Squassi, Aldo F, Molgatini, Susana L
• Format: Journal Article
• Language: English
• Published: Argentina Sociedad Argentina de Investigación Odontológica 01.12.2019
• Subjects: Adult; Aged; Aggregatibacter actinomycetemcomitans - isolation & purification; Anti-HIV Agents - pharmacology; Antiretroviral Therapy, Highly Active; Argentina; Biofilms; Dental Health Services; Dental Plaque - microbiology; Dentistry; DENTISTRY, ORAL SURGERY & MEDICINE; Female; Gingiva - microbiology; HIV Infections - complications; HIV Infections - drug therapy; HIV Infections - microbiology; Humans; Male; Middle Aged; Periodontal Diseases; Periodontal Pocket - microbiology; Periodontitis - complications; Periodontitis - microbiology; Porphyromonas gingivalis - isolation & purification; Tannerella forsythia; Treponema denticola; Argentina; HIV/AIDS; molecular biology; risk factors; periodontal diseases; biofilm; periodontitis; Periodontal diseases; Biología molecular; Enfermedad periodontal; Periodontitis; Biopelícula; Biofilm; Factores de riesgo; VIH/SIDA; Molecular biology; Risk factors
• ISSN: 1852-4834; 1852-4834

The aim of this study was to describe the microbiological profile of HIV patients under highly active antiretroviral treatment (HAART). This crosssectional study comprised 32 HIV patients with periodontal disease (PD) who had been under HAART for more than 6 months. Information about the patients' medical history was obtained from clinical records. Clinical dental examination was performed by a calibrated researcher using standard dental instruments to determine probing depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP). A total 4,765 periodontal sites were evaluated, 125 of which were also studied microbiologically. Subgingival biofilm samples were obtained using sterile paper points; one set was used for microbiological culture studies and the other for endpoint PCR. Statistical analysis was performed using KruskalWallis and posthoc DunnBonferroni contrast tests. All participants were on HAART at the time of the study, and 90.6% had a viral load below 50 copies / mm3. Prevalence of periodontally active sites was low in the study population. Microbiological studies: Black pigmented anaerobic bacteria and fusiform CFU counts were significantly higher in samples from sites with BOP and PD ≥4mm (p 0.020 and p 0.005, respectively). Molecular Assays: Detection of Porphyromonas gingivalis (p 0.002), Tannerella forsythia (p 0.023) and Treponema denticola (p 0.015) was significantly more frequent at sites with BOP and PD ≥4mm. Conclusions: The patients living with HIV/AIDS under HAART studied here had low prevalence of clinical periodontal disease signs. However, significant detection of P. gingivalis, T. denticola, and T. forsythia in periodontal active sites, and the involvement of these microorganisms as potential HIV reactivators, show the importance of creating awareness among dental health professionals of the need for close dental and periodontal monitoring in HIV patients.