Evaluation of photodynamic therapy using a diode laser and different photosensitizers against enterococcus faecalis

Photodynamic therapy (PDT) has been proven to be effective in disinfecting root canals. The aim of this present study was to evaluate the effects of PDT on the viability of Enterococcus faecalis using methylene blue (MB) and malachite green (MG) as photosensitizers. Solutions containing E. faecalis...

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Published inActa odontológica latinoamericana Vol. 27; no. 2; pp. 63 - 65
Main Authors Silva, Emmanuel J, Coutinho-Filho, Wagner P, Andrade, Aurimar O, Herrera, Daniel R, Coutinho-Filho, Tauby S, Krebs, Renato L
Format Journal Article
LanguageEnglish
Published Argentina Sociedad Argentina de Investigación Odontológica 2014
Subjects
Bacterial Load - drug effects
Dentistry
DENTISTRY, ORAL SURGERY & MEDICINE
Enterococcus faecalis - drug effects
Humans
Lasers, Semiconductor - therapeutic use
Methylene Blue - therapeutic use
Microbial Viability - drug effects
Photochemotherapy - methods
Photosensitizing Agents - therapeutic use
Root Canal Irrigants - therapeutic use
Rosaniline Dyes - therapeutic use
Sodium Hypochlorite - therapeutic use
Time Factors
Enterococcus faecalis
Endodontics
Photodynamic Therapy
Endodontia
Terapia Fotodinamica
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Abstract Photodynamic therapy (PDT) has been proven to be effective in disinfecting root canals. The aim of this present study was to evaluate the effects of PDT on the viability of Enterococcus faecalis using methylene blue (MB) and malachite green (MG) as photosensitizers. Solutions containing E. faecalis (ATCC 29212) were prepared and harvested by centrifugation to obtain cell suspensions, which were mixed with MB and MG. Samples were individually irradiated by the diode laser at a distance of 1mm for 30, 60, or 120 seconds. Colonyforming units (CFU) were determined for each treatment. PDT for 60 and 120 seconds with MG reduced E. faecalis viability significantly. Similar results were obtained when MB was used as photosensitizer. PDT using MB and MG have antibacterial effect against E. faecalis, showing potential to be used as an adjunctive antimicrobial procedure in endodontic therapy.
AbstractList Photodynamic therapy (PDT) has been proven to be effective in disinfecting root canals. The aim of this present study was to evaluate the effects of PDT on the viability of Enterococcus faecalis using methylene blue (MB) and malachite green (MG) as photosensitizers. Solutions containing E. faecalis (ATCC 29212) were prepared and harvested by centrifugation to obtain cell suspensions, which were mixed with MB and MG. Samples were individually irradiated by the diode laser at a distance of 1mm for 30, 60, or 120 seconds. Colonyforming units (CFU) were determined for each treatment. PDT for 60 and 120 seconds with MG reduced E. faecalis viability significantly. Similar results were obtained when MB was used as photosensitizer. PDT using MB and MG have antibacterial effect against E. faecalis, showing potential to be used as an adjunctive antimicrobial procedure in endodontic therapy.
Author Krebs, Renato L
Andrade, Aurimar O
Herrera, Daniel R
Coutinho-Filho, Tauby S
Coutinho-Filho, Wagner P
Silva, Emmanuel J
AuthorAffiliation Campinas State University (UNICAMP)
Grande Rio University (UNIGRANRIO)
Centro Universitário Serra dos Órgãos (UNIFESO)
Rio de Janeiro State University
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Keywords Enterococcus faecalis
Endodontics
Photodynamic Therapy
Endodontia
Terapia Fotodinamica
Language English
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SubjectTerms Bacterial Load - drug effects
Dentistry
DENTISTRY, ORAL SURGERY & MEDICINE
Enterococcus faecalis - drug effects
Humans
Lasers, Semiconductor - therapeutic use
Methylene Blue - therapeutic use
Microbial Viability - drug effects
Photochemotherapy - methods
Photosensitizing Agents - therapeutic use
Root Canal Irrigants - therapeutic use
Rosaniline Dyes - therapeutic use
Sodium Hypochlorite - therapeutic use
Time Factors
Title Evaluation of photodynamic therapy using a diode laser and different photosensitizers against enterococcus faecalis
URI https://www.ncbi.nlm.nih.gov/pubmed/25523956
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