Genetic Deletion of p90 Ribosomal S6 Kinase 2 Alters Patterns of 5-Hydroxytryptamine2A Serotonin Receptor Functional Selectivity

• Published in: Molecular pharmacology Vol. 77; no. 3; pp. 327 - 338
• Main Authors: Strachan, Ryan T., Sciaky, Noah, Cronan, Mark R., Kroeze, Wesley K., Roth, Bryan L.
• Format: Journal Article
• Language: English
• Published: The American Society for Pharmacology and Experimental Therapeutics 01.03.2010
• ISSN: 0026-895X; 1521-0111
• DOI: 10.1124/mol.109.061440

The concept of functional selectivity has now thoroughly supplanted the previously entrenched notion of intrinsic efficacy by explaining how agonists and antagonists exhibit a range of efficacies for distinct receptor-mediated responses. It is noteworthy that functional selectivity accommodates significant changes in efficacy resulting from differential expression of G protein-coupled receptor modifying proteins (i.e., “conditional efficacy”)—a phenomenon with profound implications for drug discovery. We have uncovered a novel regulatory mechanism whereby p90 ribosomal S6 kinase 2 (RSK2) interacts with 5-hydroxytryptamine 2A (5-HT 2A ) serotonin receptors and attenuates receptor signaling via direct receptor phosphorylation ( Proc Natl Acad Sci U S A 103: 4717–4722, 2006; J Biol Chem 284: 5557–5573, 2009). This discovery, together with the mounting evidence for conditional efficacy, suggested to us that 5-HT 2A agonist signaling might be disproportionately affected by alterations in RSK2 expression. To test this hypothesis, we evaluated a chemically diverse set of 5-HT 2A agonists at three readouts of 5-HT 2A receptor activation in both wild-type (WT) and RSK2 knock-out (KO) mouse embryonic fibroblasts (MEFs). Here we report that 5-HT 2A receptor agonist efficacies were significantly and variably augmented in RSK2 KO MEFs compared with WT MEFs. As a result, relative agonist efficacies were significantly altered, and even reversed, between WT and RSK2 KO MEFs for a single effector readout. This study provides the first evidence that deletion of a single kinase can elicit profound changes in patterns of agonist functional selectivity.