Preparation of selectively decellular xenoskin and its biocompatibility

• Published in: Zhongguo xiu fu chong jian wai ke za zhi Vol. 24; no. 6; p. 668
• Main Authors: Sun, Tianjun, Chai, Jiake, Han, Yanfu, Yang, Hongming, Liu, Lingying, Su, Peng, Feng, Rui
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2010
• Subjects: Animals; Dermis - cytology; Dermis - transplantation; Epidermis - cytology; Epidermis - transplantation; Extracellular Matrix; Materials Testing; Rats; Rats, Wistar; Skin Transplantation; Swine
• ISSN: 1002-1892

To prepare and study the biocompatibility of selectively decellular xenoskin which has the character of the lower antigen, continuous epidermis, and the dermal matrix without any cellular components. The porcine skin was treated with glutaraldehyde solution, trypsin, and detergent solution TritonX-100 to prepare the selectively decellular xenoskin. The cytotoxicity was tested according to GB/T16886.5-2003 biological evaluation of medical devices for in vitro cytotoxicity, and the levels of cytotoxicity were evaluated with the United States Pharmacopeia. Subdermal implantation was tested according to GB/T16886.6-1997 biological evaluation of medical devices for local effects after implantation. Seventy-two mature Wistar rats were randomly assigned to groups A, B, and C (n = 24). Three kinds of materials were implanted into subcutaneous of rats back. Selectively decellular xenoskin was transplanted into group A, fresh porcine skin was transplanted into group B, and allogeneic skin was transplanted into group C.