Simultaneous determination of nicotinamide and N1‐methylnicotinamide in human serum by LC–MS/MS to associate their serum concentrations with obesity

• Published in: Biomedical chromatography Vol. 36; no. 2; pp. e5261 - n/a
• Main Authors: Chu, Jihong, Liu, Ming, Dai, Guoliang, Li, Changyin, Wu, Ting, Zou, Jiandong, Ju, Wenzheng, Xu, Meijuan
• Format: Journal Article
• Language: English
• Published: 01.02.2022
• Subjects: LC–MS/MS; N1‐methyl; nicotinamide; obesity

A rapid and sensitive LC–MS/MS method was developed and validated for the simultaneous determination of nicotinamide and its metabolite N1‐methylnicotinamide in human serum. Serum samples were prepared by protein precipitation with acetonitrile. The chromatographic separation was performed on a Wate...