VLK drives extracellular phosphorylation of EphB2 to govern the EphB2-NMDAR interaction and injury-induced pain

Phosphorylation of hundreds of protein extracellular domains is mediated by two kinase families, yet the significance of these kinases is underexplored. Here, we find that the presynaptic release of the tyrosine directed-ectokinase, Vertebrate Lonesome Kinase (VLK/Pkdcc), is necessary and sufficient...

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Published inbioRxiv
Main Authors Srikanth, Kolluru D, Elahi, Hajira, Chander, Praveen, Washburn, Halley R, Hassler, Shayne, Mwirigi, Juliet M, Kume, Moeno, Loucks, Jessica, Arjarapu, Rohita, Hodge, Rachel, Shiers, Stephanie I, Sankaranarayanan, Ishwarya, Erdjument-Bromage, Hediye, Neubert, Thomas A, Campbell, Zachary T, Paik, Raehum, Price, Theodore J, Dalva, Matthew B
Format Journal Article
LanguageEnglish
Published United States 18.03.2024
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Summary:Phosphorylation of hundreds of protein extracellular domains is mediated by two kinase families, yet the significance of these kinases is underexplored. Here, we find that the presynaptic release of the tyrosine directed-ectokinase, Vertebrate Lonesome Kinase (VLK/Pkdcc), is necessary and sufficient for the direct extracellular interaction between EphB2 and GluN1 at synapses, for phosphorylation of the ectodomain of EphB2, and for injury-induced pain. is an essential gene in the nervous system, and VLK is found in synaptic vesicles, and is released from neurons in a SNARE-dependent fashion. VLK is expressed by nociceptive sensory neurons where presynaptic sensory neuron-specific knockout renders mice impervious to post-surgical pain, without changing proprioception. VLK defines an extracellular mechanism that regulates protein-protein interaction and non-opioid-dependent pain in response to injury.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Working Paper/Pre-Print-1
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ISSN:2692-8205
2692-8205
DOI:10.1101/2024.03.18.585314