TurboFlow在线净化-液相色谱-串联质谱法快速检测人尿中鹅膏肽类毒素
O658; 鹅膏肽类毒素是一类环状多肽类蘑菇毒素,中毒后会造成急性肝损伤,病死率非常高.我国因误食野生毒蘑菇导致的中毒事件常有发生,测定人尿中鹅膏肽类毒素的浓度,可为临床早期诊断和救治提供有价值的信息.该研究建立了TurboFlow (TF)在线净化-液相色谱-三重四极杆质谱快速定量检测尿液中5种鹅膏肽类毒素(α-鹅膏毒肽、β-鹅膏毒肽、γ-鹅膏毒肽、羧基二羟鬼笔毒肽和二羟鬼笔毒肽)的新方法.尿液样品经高速离心后,直接注入TurboFlow在线净化-液相色谱-串联质谱进行分析.对影响TF在线净化的参数如TF净化柱、上样溶剂、洗脱溶剂、转移流速、转移时间等进行了优化.在优化后的实验条件下,以Tu...
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| Published in | Sepu Vol. 39; no. 3; pp. 338 - 345 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | Chinese English |
| Published |
No. 457, Zhongshan Road, Shahekou District, Dalian City, Liaoning Province
浙江省海产品健康危害因素关键技术研究重点实验室(舟山市疾病预防控制中心),浙江舟山316021%岱山县疾病预防控制中心,浙江岱山 316200
08.03.2021
Editorial board of Chinese Journal of Chromatography |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-8713 |
| DOI | 10.3724/SP.J.1123.2020.06005 |
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| Abstract | O658; 鹅膏肽类毒素是一类环状多肽类蘑菇毒素,中毒后会造成急性肝损伤,病死率非常高.我国因误食野生毒蘑菇导致的中毒事件常有发生,测定人尿中鹅膏肽类毒素的浓度,可为临床早期诊断和救治提供有价值的信息.该研究建立了TurboFlow (TF)在线净化-液相色谱-三重四极杆质谱快速定量检测尿液中5种鹅膏肽类毒素(α-鹅膏毒肽、β-鹅膏毒肽、γ-鹅膏毒肽、羧基二羟鬼笔毒肽和二羟鬼笔毒肽)的新方法.尿液样品经高速离心后,直接注入TurboFlow在线净化-液相色谱-串联质谱进行分析.对影响TF在线净化的参数如TF净化柱、上样溶剂、洗脱溶剂、转移流速、转移时间等进行了优化.在优化后的实验条件下,以TurboFlowTM Cyclone柱(50 mm×0.5 mm)为净化柱,Hypersil GOLD C18柱(100 mm×2.1 mm)为分析柱,甲醇和4 mmol/L乙酸铵为流动相进行梯度洗脱,电喷雾正离子选择反应监测(SRM)模式下进行检测,基质匹配外标法定量.结果 表明,鹅膏肽类毒素在1.0～50.0μg/L范围内呈现良好的线性关系,相关系数均可达到0.997以上.方法 的检出限为0.15～0.3 μg/L,定量限为0.5～ 1.0 μg/L.在2.0、5.0和10.0μg/L的加标水平下,5种鹅膏肽类毒素的日内和日间回收率分别为87.0％～108.6％和86.8％ ～ 112.7％,日内、日间相对标准偏差(RSD)均小于14.5％.该检测方法准确、快速、灵敏度高、易操作,适用于公共卫生应急检测或临床中毒病因识别检测. |
|---|---|
| AbstractList | 鹅膏肽类毒素是一类环状多肽类蘑菇毒素,中毒后会造成急性肝损伤,病死率非常高。我国因误食野生毒蘑菇导致的中毒事件常有发生,测定人尿中鹅膏肽类毒素的浓度,可为临床早期诊断和救治提供有价值的信息。该研究建立了TurboFlow (TF)在线净化-液相色谱-三重四极杆质谱快速定量检测尿液中5种鹅膏肽类毒素(
α
-鹅膏毒肽、
β
-鹅膏毒肽、
γ
-鹅膏毒肽、羧基二羟鬼笔毒肽和二羟鬼笔毒肽)的新方法。尿液样品经高速离心后,直接注入TurboFlow在线净化-液相色谱-串联质谱进行分析。对影响TF在线净化的参数如TF净化柱、上样溶剂、洗脱溶剂、转移流速、转移时间等进行了优化。在优化后的实验条件下,以TurboFlow
TM
Cyclone柱(50 mm×0.5 mm)为净化柱,Hypersil GOLD C
18
柱(100 mm×2.1 mm)为分析柱,甲醇和4 mmol/L乙酸铵为流动相进行梯度洗脱,电喷雾正离子选择反应监测(SRM)模式下进行检测,基质匹配外标法定量。结果表明,鹅膏肽类毒素在1.0~50.0 μg/L范围内呈现良好的线性关系,相关系数均可达到0.997以上。方法的检出限为0.15~0.3 μg/L,定量限为0.5~1.0 μg/L。在2.0、5.0和10.0 μg/L的加标水平下,5种鹅膏肽类毒素的日内和日间回收率分别为87.0%~108.6%和86.8%~112.7%,日内、日间相对标准偏差(RSD)均小于14.5%。该检测方法准确、快速、灵敏度高、易操作,适用于公共卫生应急检测或临床中毒病因识别检测。 O658; 鹅膏肽类毒素是一类环状多肽类蘑菇毒素,中毒后会造成急性肝损伤,病死率非常高.我国因误食野生毒蘑菇导致的中毒事件常有发生,测定人尿中鹅膏肽类毒素的浓度,可为临床早期诊断和救治提供有价值的信息.该研究建立了TurboFlow (TF)在线净化-液相色谱-三重四极杆质谱快速定量检测尿液中5种鹅膏肽类毒素(α-鹅膏毒肽、β-鹅膏毒肽、γ-鹅膏毒肽、羧基二羟鬼笔毒肽和二羟鬼笔毒肽)的新方法.尿液样品经高速离心后,直接注入TurboFlow在线净化-液相色谱-串联质谱进行分析.对影响TF在线净化的参数如TF净化柱、上样溶剂、洗脱溶剂、转移流速、转移时间等进行了优化.在优化后的实验条件下,以TurboFlowTM Cyclone柱(50 mm×0.5 mm)为净化柱,Hypersil GOLD C18柱(100 mm×2.1 mm)为分析柱,甲醇和4 mmol/L乙酸铵为流动相进行梯度洗脱,电喷雾正离子选择反应监测(SRM)模式下进行检测,基质匹配外标法定量.结果 表明,鹅膏肽类毒素在1.0～50.0μg/L范围内呈现良好的线性关系,相关系数均可达到0.997以上.方法 的检出限为0.15～0.3 μg/L,定量限为0.5～ 1.0 μg/L.在2.0、5.0和10.0μg/L的加标水平下,5种鹅膏肽类毒素的日内和日间回收率分别为87.0％～108.6％和86.8％ ～ 112.7％,日内、日间相对标准偏差(RSD)均小于14.5％.该检测方法准确、快速、灵敏度高、易操作,适用于公共卫生应急检测或临床中毒病因识别检测. |
| Author | 余新威 方为 邱凤梅 |
| AuthorAffiliation | 浙江省海产品健康危害因素关键技术研究重点实验室(舟山市疾病预防控制中心),浙江舟山316021%岱山县疾病预防控制中心,浙江岱山 316200 |
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| Author_FL | YU Xinwei FANG Li QIU Fengmei |
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| Keywords | 尿液 在线净化 蘑菇毒素 鹅膏肽类毒素 液相色谱-串联质谱法 |
| Language | Chinese English |
| License | 本文是开放获取文章，遵循CC BY 4.0协议 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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| Publisher | 浙江省海产品健康危害因素关键技术研究重点实验室(舟山市疾病预防控制中心),浙江舟山316021%岱山县疾病预防控制中心,浙江岱山 316200 Editorial board of Chinese Journal of Chromatography |
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| Snippet | O658; 鹅膏肽类毒素是一类环状多肽类蘑菇毒素,中毒后会造成急性肝损伤,病死率非常高.我国因误食野生毒蘑菇导致的中毒事件常有发生,测定人尿中鹅膏肽类毒素的浓度,可为临床早期诊断和救治提供有价值的信息.该研究建立了TurboFlow... 鹅膏肽类毒素是一类环状多肽类蘑菇毒素,中毒后会造成急性肝损伤,病死率非常高。我国因误食野生毒蘑菇导致的中毒事件常有发生,测定人尿中鹅膏肽类毒素的浓度,可为临床早期诊断和救治提供有价值的信息。该研究建立了TurboFlow (TF)在线净化-液相色谱-三重四极杆质谱快速定量检测尿液中5种鹅膏肽类毒素( α -鹅膏毒肽、... |
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| Title | TurboFlow在线净化-液相色谱-串联质谱法快速检测人尿中鹅膏肽类毒素 |
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