High Resolution CZE-MS Quantitative Characterization of Intact Biopharmaceutical Proteins: Proteoforms of Interferon- beta 1

• Published in: Analytical chemistry (Washington) Vol. 88; no. 2; p. 1138
• Main Authors: Bush, David R, Zang, Li, Belov, Arseniy M, Ivanov, Alexander R, Karger, Barry L
• Format: Journal Article
• Language: English
• Published: 01.01.2016
• Subjects: Charge; Columns (structural); Glycan; High resolution; Isomers; Proteins; Quantitative analysis; Separation

New and improved methods are required for the enhanced characterization of complex biopharmaceuticals, especially those with charge and glycan heterogeneity. High resolution separation and mass spectrometry (MS) analysis of intact proteoforms can contribute significantly to the characterization of s...