Nucleic acid molecular biomarkers for diagnostic biodosimetry applications: use of the fluorogenic 5'-nuclease polymerase chain reaction assay

• Published in: Military medicine Vol. 167; no. 2 Suppl; p. 16
• Main Authors: Blakely, William F, Miller, Alexandra C, Luo, Lei, Lukas, Jason, Hornby, Zachary D, Hamel, Christine J C, Nelson, Joseph T, Escalada, Nestor E, Prasanna, Pataje G S
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.02.2002
• Subjects: Acids; Biomarkers; Gene expression; Humans; Laboratories; Lymphocytes - radiation effects; Mitochondrial DNA; Mutation; Polymerase Chain Reaction; Radiation; Radiation Dosage; X-rays

A reliable, relatively easy method for diagnostic assessment of radiation exposure is needed to support the triage of radiation casualties and medical treatment decisions in military defense operations. Our strategy is to identify radiation-responsive DNA mutations and gene expression targets that c...