Sensitive, Efficient Quantitation of 13C-Enriched Nucleic Acids via Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry for Applications in Stable Isotope Probing

• Published in: Applied and Environmental Microbiology Vol. 80; no. 23; pp. 7206 - 7211
• Main Authors: Wilhelm, Roland, Szeitz, András, Klassen, Tara L, Mohn, William W
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.12.2014
• Subjects: Carbon Isotopes - analysis; Chromatography, High Pressure Liquid - methods; Isotope Labeling; Mass Spectrometry - methods; Methods; Nucleic Acids - analysis

Stable isotope probing (SIP) of nucleic acids is a powerful tool for studying the functional traits of microbial populations within complex communities, but SIP involves a number of technical challenges. Many of the difficulties in DNA-SIP and RNA-SIP experiments can be effectively overcome with an...