Experimental murine model of renal cancer

• Published in: Actas urologicas españolas Vol. 41; no. 7; pp. 445 - 450
• Main Authors: Padilla-Fernández, B, García-Cenador, M B, Rodríguez-Marcos, P, López-Marcos, J F, Antúnez-Plaza, P, Silva-Abuín, J M, López-Montañés, D, García-Criado, F J, Lorenzo-Gómez, M F
• Format: Journal Article
• Language: English; Spanish
• Published: Spain 01.09.2017
• Subjects: Animals; Disease Models, Animal; Kidney Neoplasms; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Validation; Carcinoma renal; Modelo experimental; Renal carcinoma; Experimental model; Validación
• ISSN: 1699-7980
• DOI: 10.1016/j.acuro.2016.11.005

The objective of this study was to determine the reproducibility in a murine model of renal tumours of various histological strains that could be useful for investigating the response to target drugs. Development and analysis of the "in vivo" model: tumour xenograft of renal cell carcinomas with Balb/c nude athymic mice. Nontumourous human renal tissue was implanted in the interscapular region of 5 mice, chromophobe renal cell carcinoma was implanted in 5 mice (which, after checking its growth, was prepared for implantation in another 10 mice) and Fuhrman grade 2 clear cell renal cell carcinoma (CCRCC) was implanted in 5 mice (which was also subsequently implanted in 10 mice). We monitored the tumour size, onset of metastases and increase in size and number of tumours. When the size had reached a point greater than or equal to locally advanced or metastatic carcinoma, the animals were euthanised for a pathological and immunohistochemical study and a second phase of implantation. The subcutaneous xenograft of the healthy tissue did not grow. The animals were euthanised at 6 months and no renal tissue was found. The chromophobe renal cell carcinoma cells grew in the initial phase (100%); however, in the second phase, we observed a chronic lymphomonocyte inflammatory reaction and a foreign body reaction. The CCRCC grew at 5-8 months both in the first and second phase (100%), maintaining the tumour type and grade. The model with athymic Balb/c nude mice is useful for reproducing CCRCC, with the same histological characteristics and aggressiveness as native human tumours, promoting the development of the second experimental phase.