Insights into the inhibitory mechanisms of the regulatory protein IIA(Glc) on melibiose permease activity

• Published in: The Journal of biological chemistry Vol. 289; no. 47; p. 33012
• Main Authors: Hariharan, Parameswaran, Guan, Lan
• Format: Journal Article
• Language: English
• Published: United States 21.11.2014
• Subjects: Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Binding Sites - genetics; Calorimetry - methods; Electrophoresis, Polyacrylamide Gel; Entropy; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Glucose - metabolism; Kinetics; Melibiose - metabolism; Models, Molecular; Mutation; Phosphoenolpyruvate Sugar Phosphotransferase System - chemistry; Phosphoenolpyruvate Sugar Phosphotransferase System - genetics; Phosphoenolpyruvate Sugar Phosphotransferase System - metabolism; Protein Binding; Protein Structure, Tertiary; Salmonella typhimurium - enzymology; Salmonella typhimurium - genetics; Species Specificity; Symporters - chemistry; Symporters - genetics; Symporters - metabolism; Energetics; Melibiose Permease; Thermodynamics; IIAGlc Binding; Protein-Protein Interaction; Carbohydrate Metabolism; Isothermal Titration Calorimetry (ITC); Sugar Binding; PTS

The phosphotransfer protein IIA(Glc) of the bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system plays a key role in the regulation of carbohydrate metabolism. Melibiose permease (MelB) is one among several permeases subject to IIA(Glc) regulation. The regulatory mechanisms are poorl...