Improving zero-mode waveguide structure for enhancing signal-to-noise ratio of real-time single-molecule fluorescence imaging: a computational study

• Published in: Physical review. E, Statistical, nonlinear, and soft matter physics Vol. 88; no. 1; p. 012727
• Main Authors: Tanii, Takashi, Akahori, Rena, Higano, Shun, Okubo, Kotaro, Yamamoto, Hideaki, Ueno, Taro, Funatsu, Takashi
• Format: Journal Article
• Language: English
• Published: United States 01.07.2013
• Subjects: Computer Simulation; Fluorescence; Models, Theoretical; Molecular Imaging - methods; Signal-To-Noise Ratio

We investigated the signal-to-noise ratio (S/N) of real-time single-molecule fluorescence imaging (SMFI) using zero-mode waveguides (ZMWs). The excitation light and the fluorescence propagating from a molecule in the ZMW were analyzed by computational optics simulation. The dependence of the S/N on...