OCT4 activity during conversion of human intermediately reprogrammed stem cells to iPSCs through mesenchymal-epithelial transition

• Published in: Development (Cambridge) Vol. 143; no. 1; pp. 15 - 23
• Main Authors: Teshigawara, Rika, Hirano, Kunio, Nagata, Shogo, Ainscough, Justin, Tada, Takashi
• Format: Journal Article
• Language: English
• Published: England 01.01.2016
• Subjects: Cell Division - physiology; Cell Line; Cell Transdifferentiation - physiology; Cellular Reprogramming - physiology; Clustered Regularly Interspaced Short Palindromic Repeats; Enzyme Activation; Epithelial Cells - cytology; Green Fluorescent Proteins - genetics; Homeodomain Proteins - metabolism; Humans; Induced Pluripotent Stem Cells - cytology; Kruppel-Like Transcription Factors - genetics; Kruppel-Like Transcription Factors - metabolism; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Nanog Homeobox Protein; Octamer Transcription Factor-3 - genetics; Octamer Transcription Factor-3 - metabolism; Proto-Oncogene Proteins c-myc - genetics; Proto-Oncogene Proteins c-myc - metabolism; SOXB1 Transcription Factors - genetics; SOXB1 Transcription Factors - metabolism; Human; OCT4; CRISPR; Reprogramming; IPS; MET

To facilitate understanding the mechanisms of somatic reprogramming to human induced pluripotent stem cells (iPSCs), we have established intermediately reprogrammed stem cells (iRSCs), human mesenchymal cells that express exogenous Oct4, Sox2, Klf4 and c-Myc (OSKM) and endogenous SOX2 and NANOG. iRS...