Nocardioides agri sp. nov., isolated from garden soil
A novel bacterial strain, designated as MAH-18 , was isolated from soil sampled in a flower garden. Cells of strain MAH-18 were Gram-stain-positive, aerobic, motile, and rod-shaped. The colonies were beige in colour, smooth, and spherical when grown on Reasoner's 2A agar medium. Strain MAH-18 g...
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Published in | International journal of systematic and evolutionary microbiology Vol. 74; no. 6 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
01.06.2024
|
Subjects | |
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Abstract | A novel bacterial strain, designated as MAH-18
, was isolated from soil sampled in a flower garden. Cells of strain MAH-18
were Gram-stain-positive, aerobic, motile, and rod-shaped. The colonies were beige in colour, smooth, and spherical when grown on Reasoner's 2A agar medium. Strain MAH-18
grew at 20-40 °C, pH 6.0-8.0, and 0-1.0 % NaCl. Cells were able to hydrolyse aesculin, gelatin, and Tween 20. According to the 16S rRNA gene sequence comparisons, the isolate was determined to be a member of the genus
and most closely related to
OS4
(97.9 %),
DS-30
(97.9 %),
GW-9
(97.6 %),
mbc-2
(97.5 %),
HWE 2-02
(97.4 %), and
GBK3QG-3
(96.3 %). Strain MAH-18
has a draft genome size of 4 788 325 bp (eight contigs), 4572 protein-coding genes, 46 tRNA, and three rRNA genes. The average nucleotide identity and digital DNA-DNA hybridization values between strain MAH-18
and the closest type strains were 81.5-83.4 % and 24.4-25.8 %, respectively.
genome mining revealed several biosynthetic gene clusters in the genome of the novel strain MAH-18
. The G+C content of the genomic DNA of strain was 72.2 mol% and the predominant isoprenoid quinone was MK-8 (H
). The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and unknown phospholipids. The major cellular fatty acids were determined to be C
iso and C
6
. The DNA-DNA hybridization results and phenotypic, genotypic, and chemotaxonomic data demonstrated that strain MAH-18
represents a novel species, for which the name
sp. nov. is proposed, with MAH-18
as the type strain (=KACC 19744
=CGMCC 1.13656
). |
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AbstractList | A novel bacterial strain, designated as MAH-18
, was isolated from soil sampled in a flower garden. Cells of strain MAH-18
were Gram-stain-positive, aerobic, motile, and rod-shaped. The colonies were beige in colour, smooth, and spherical when grown on Reasoner's 2A agar medium. Strain MAH-18
grew at 20-40 °C, pH 6.0-8.0, and 0-1.0 % NaCl. Cells were able to hydrolyse aesculin, gelatin, and Tween 20. According to the 16S rRNA gene sequence comparisons, the isolate was determined to be a member of the genus
and most closely related to
OS4
(97.9 %),
DS-30
(97.9 %),
GW-9
(97.6 %),
mbc-2
(97.5 %),
HWE 2-02
(97.4 %), and
GBK3QG-3
(96.3 %). Strain MAH-18
has a draft genome size of 4 788 325 bp (eight contigs), 4572 protein-coding genes, 46 tRNA, and three rRNA genes. The average nucleotide identity and digital DNA-DNA hybridization values between strain MAH-18
and the closest type strains were 81.5-83.4 % and 24.4-25.8 %, respectively.
genome mining revealed several biosynthetic gene clusters in the genome of the novel strain MAH-18
. The G+C content of the genomic DNA of strain was 72.2 mol% and the predominant isoprenoid quinone was MK-8 (H
). The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and unknown phospholipids. The major cellular fatty acids were determined to be C
iso and C
6
. The DNA-DNA hybridization results and phenotypic, genotypic, and chemotaxonomic data demonstrated that strain MAH-18
represents a novel species, for which the name
sp. nov. is proposed, with MAH-18
as the type strain (=KACC 19744
=CGMCC 1.13656
). |
Author | Huq, Md Amdadul Rahman, M Mizanur Parvez, Md Anowar Khasru Nam, Kihong Akter, Shahina Rahman, Md Shahedur Kang, Kwon-Kyoo |
Author_xml | – sequence: 1 givenname: Md Amdadul surname: Huq fullname: Huq, Md Amdadul organization: Department of Food and Nutrition, Chung-Ang University, Anseong-si, Gyeonggi-do, 17546, Republic of Korea – sequence: 2 givenname: Kihong surname: Nam fullname: Nam, Kihong organization: Department of Horticultural Life Science, Hankyong National University, Anseong-si, Gyeonggi-do, 17579, Republic of Korea – sequence: 3 givenname: Md Shahedur surname: Rahman fullname: Rahman, Md Shahedur organization: Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore 7408, Bangladesh – sequence: 4 givenname: M Mizanur surname: Rahman fullname: Rahman, M Mizanur organization: Department of Biotechnology and Genetic Engineering, Faculty of Biological Science, Islamic University, Kushtia-7003, Bangladesh – sequence: 5 givenname: Md Anowar Khasru surname: Parvez fullname: Parvez, Md Anowar Khasru organization: Department of Microbiology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh – sequence: 6 givenname: Kwon-Kyoo surname: Kang fullname: Kang, Kwon-Kyoo organization: Department of Horticultural Life Science, Hankyong National University, Anseong-si, Gyeonggi-do, 17579, Republic of Korea – sequence: 7 givenname: Shahina surname: Akter fullname: Akter, Shahina organization: Department of Food Science and Biotechnology, Gachon University, Seongnam, 461-701, Republic of Korea |
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Snippet | A novel bacterial strain, designated as MAH-18
, was isolated from soil sampled in a flower garden. Cells of strain MAH-18
were Gram-stain-positive, aerobic,... |
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SubjectTerms | Actinomycetales - classification Actinomycetales - genetics Actinomycetales - isolation & purification Bacterial Typing Techniques Base Composition DNA, Bacterial - genetics Fatty Acids Gardens Genome, Bacterial Nucleic Acid Hybridization Phospholipids Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Soil Microbiology |
Title | Nocardioides agri sp. nov., isolated from garden soil |
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