Quantitation of amyloid beta peptides Aβ(1-38), Aβ(1-40), and Aβ(1-42) in human cerebrospinal fluid by ultra-performance liquid chromatography-tandem mass spectrometry

• Published in: Analytical biochemistry Vol. 419; no. 2; p. 133
• Main Authors: Lame, Mary E, Chambers, Erin E, Blatnik, Matthew
• Format: Journal Article
• Language: English
• Published: United States 15.12.2011
• Subjects: Amino Acid Sequence; Amyloid beta-Peptides - cerebrospinal fluid; Amyloid beta-Peptides - chemistry; Chromatography, High Pressure Liquid - methods; Humans; Molecular Sequence Data; Peptide Fragments - cerebrospinal fluid; Peptide Fragments - chemistry; Quality Control; Reference Standards; Solid Phase Extraction; Tandem Mass Spectrometry - methods

Critical events in Alzheimer's disease (AD) involve an imbalance between the production and clearance of amyloid beta (Aβ) peptides from the brain. Current methods for Aβ quantitation rely heavily on immuno-based techniques. However, these assays require highly specific antibodies and reagents...