Detection by flow cytometry of T cell subsets secreting IL-2 and UFNy(Gamma): optimalisation for the technic and the establishment of reference values

• Published in: Pathologie biologie (Paris) Vol. 55; no. 5; p. 222
• Main Authors: Kallel, C, Masy, E, Duthilleul, P
• Format: Journal Article
• Language: French
• Published: France 01.06.2007
• Subjects: Adult; Antigens, CD - analysis; CD4-Positive T-Lymphocytes - immunology; Cell Culture Techniques; Cell Membrane - immunology; Cytokines - metabolism; Flow Cytometry - methods; Fluorescein-5-isothiocyanate; Humans; Interferon-gamma - secretion; Interleukin-2 - secretion; Ionomycin - pharmacology; Kinetics; Reference Values; T-Lymphocyte Subsets - cytology; T-Lymphocyte Subsets - drug effects; T-Lymphocyte Subsets - immunology; Tetradecanoylphorbol Acetate - pharmacology

A dysregulation in Th1/Th2 balance has been described for different pathological situations. Knowing the cytokine profile in a given pathology could assist in understanding the disease mechanism and in choosing an immune intervention most effective for the management of this condition. In this work,...