P2y(12), a new platelet ADP receptor, target of clopidogrel

• Published in: Biochemical and biophysical research communications Vol. 283; no. 2; p. 379
• Main Authors: Savi, P, Labouret, C, Delesque, N, Guette, F, Lupker, J, Herbert, J M
• Format: Journal Article
• Language: English
• Published: United States 04.05.2001
• Subjects: Adenosine Diphosphate - analogs & derivatives; Adenosine Diphosphate - metabolism; Adenylyl Cyclases - metabolism; Animals; Blood Platelets - drug effects; Blood Platelets - metabolism; CHO Cells; Cricetinae; Down-Regulation; Humans; Kinetics; Receptors, Purinergic P2 - blood; Receptors, Purinergic P2 - drug effects; Receptors, Purinergic P2 - genetics; Ticlopidine - analogs & derivatives; Ticlopidine - pharmacology; Transfection

The binding characteristics of (33)P-2MeS-ADP, a stable analogue of ADP, were determined on CHO cells transfected with the human P2Y(12) receptor, a novel purinergic receptor. These transfected CHO cells displayed a strong affinity for (33)P-2MeS-ADP, the binding characteristics of which corresponde...