Diagnosis of immediate-type beta-lactam allergy in vitro by flow-cytometric basophil activation test and sulfidoleukotriene production: a multicenter study

• Published in: Journal of investigational allergology & clinical immunology Vol. 19; no. 2; pp. 91 - 109
• Main Authors: De Week, A L, Sanz, M L, Gamboa, P M, Aberer, W, Sturm, G, Bilo, M B, Montroni, M, Blanca, M, Torres, M J, Mayorga, L, Campi, P, Manfredi, M, Drouet, M, Sainte-Laudy, J, Romano, A, Merk, H, Weber, J M, Jermann, T M
• Format: Journal Article
• Language: English
• Published: Spain 2009
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Basophil Degranulation Test; beta-Lactams - administration & dosage; beta-Lactams - immunology; Cell Separation; Drug Hypersensitivity - blood; Drug Hypersensitivity - diagnosis; Drug Hypersensitivity - immunology; Drug Hypersensitivity - physiopathology; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Humans; Immunoglobulin E - blood; Leukotrienes - immunology; Leukotrienes - metabolism; Male; Middle Aged; Sensitivity and Specificity; Skin Tests; Sulfides - immunology; Sulfides - metabolism

This multicenter study aimed to evaluate the diagnostic value of 2 cellular tests based on basophil reactivity--the basophil activation test (BAT, Flow-CAST) and the sulfidoleukotriene release assay (CAST-ELISA)--in immediate-type beta-lactam allergy, particularly in patients with a clinical history...