The role of plasmatic erythrocytic membrane in formation of hypoxia in patients with chronic cardiac failure

• Published in: Terapevtic̆eskii arhiv Vol. 77; no. 9; p. 70
• Main Authors: Maksimov, G V, Rodnenkov, O V, Luneva, O G, Brazhe, N A, Parshina, E Iu, Rubin, A B, Chazov, E I
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2005
• Subjects: Adult; Aged; Cardiac Output, Low - complications; Cell Membrane - chemistry; Chronic Disease; Electron Spin Resonance Spectroscopy; Erythrocyte Membrane - chemistry; Erythrocytes - cytology; Hematoporphyrins - chemistry; Hemoglobins - analysis; Hemoglobins - metabolism; Humans; Hypoxia - diagnosis; Hypoxia - etiology; Ligands; Male; Middle Aged; Nitric Oxide - analysis; Oxygen Consumption; Oxyhemoglobins - analysis; Protein Conformation; Viscosity
• ISSN: 0040-3660

To study oxygen supply to erythrocytes in patients with chronic cardiac failure as a factor provoking hypoxia. The trial enrolled 12 men with chronic cardiac failure (CCF) of functional NYHA class II aged 37 to 74 years (group A). Group B--healthy donors (n = 10) aged 20-40 years. Conformation changes of hemoporphirine were detected with degradation spectroscopy, viscosity of plasmatic membrane--with resonance spectroscopy. Degradation spectroscopy estimated content of Hb-ligant complexes as 0.34 +/- 0.013; complexes I Hb-NO 0.73 +/- 0.11, complexes II Hb-NO 0.28 +/- 0.08 (group A). Electronic-paramagnetic spectroscopy has estimated: S--0.716 +/- 0.005 (group A), 0.667 +/- 0.011 (group B). In group B- 0.5 +/- 0.08; 0.41 +/- 0.22; 0.55 +/- 0.12, respectively. Patients with CHF have high microviscosity of plasmatic erythrocytic membrane near location of polar lipid groups; subnormal content of oxyhemoglobin and elevation of the number of complexes of nitric hemoglobin-oxide; altered binding of nitric oxide with hemoglobin.