Microsatellites for Phytolacca acinosa (Phytolaccaceae), a traditional medicinal herb1
Premise of the study: Phytolacca acinosa (Phytolaccaceae) is a traditional Chinese herb with multiple medicinal uses and is an important pigment source. Due to excessive human harvesting, the population numbers and sizes have decreased dramatically. Methods and Results: Using an enriched genomic lib...
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Published in | Applications in plant sciences Vol. 5; no. 10 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Botanical Society of America
19.10.2017
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Subjects | |
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Abstract | Premise of the study:
Phytolacca acinosa (Phytolaccaceae) is a traditional Chinese herb with multiple medicinal uses and is an important pigment source. Due to excessive human harvesting, the population numbers and sizes have decreased dramatically.
Methods and Results:
Using an enriched genomic library, we developed and characterized 15 microsatellite primers for P. acinosa, 13 of which were polymorphic. The number of alleles varied from two to seven. The observed heterozygosity and expected heterozygosity per locus ranged from 0.267 to 1.000 and 0.331 to 0.743, respectively. All of the primers that were developed were also successfully applied in P. americana.
Conclusions:
These markers should be useful in probing the genetic diversity, genetic structure, and mating systems of P. acinosa, which could provide information about protecting and sustainably harvesting this species. |
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AbstractList | Premise of the study:
Phytolacca acinosa (Phytolaccaceae) is a traditional Chinese herb with multiple medicinal uses and is an important pigment source. Due to excessive human harvesting, the population numbers and sizes have decreased dramatically.
Methods and Results:
Using an enriched genomic library, we developed and characterized 15 microsatellite primers for P. acinosa, 13 of which were polymorphic. The number of alleles varied from two to seven. The observed heterozygosity and expected heterozygosity per locus ranged from 0.267 to 1.000 and 0.331 to 0.743, respectively. All of the primers that were developed were also successfully applied in P. americana.
Conclusions:
These markers should be useful in probing the genetic diversity, genetic structure, and mating systems of P. acinosa, which could provide information about protecting and sustainably harvesting this species. |
Author | Cheng, Yueqin Li, Pingping Zhang, Jianhang Yan, Fengming Wang, Hongwei Yang, Yi |
Author_xml | – sequence: 1 givenname: Yueqin surname: Cheng fullname: Cheng, Yueqin – sequence: 2 givenname: Pingping surname: Li fullname: Li, Pingping – sequence: 3 givenname: Yi surname: Yang fullname: Yang, Yi – sequence: 4 givenname: Jianhang surname: Zhang fullname: Zhang, Jianhang – sequence: 5 givenname: Fengming surname: Yan fullname: Yan, Fengming – sequence: 6 givenname: Hongwei surname: Wang fullname: Wang, Hongwei email: whwcas@163.com |
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Copyright | 2017 The Author(s) 2017 Cheng et al. Published by the Botanical Society of America 2017 |
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References | 2009; 11 1990; 2 2010; 10 2009; 25 2016; 6 2000; 18 2015; 3 2011; 17 2002; 21 2014; 42 |
References_xml | – volume: 18 start-page: 233 year: 2000 end-page: 234 article-title: An economic method for the fluorescent labeling of PCR fragments publication-title: Nature Biotechnology – volume: 2 start-page: 13 year: 1990 end-page: 16 article-title: Effects of polysaccharides I on cytotoxicity of macrophages and its production of tumor necrosis factor and interleukin 1 publication-title: Chinese Journal of Cancer Research – volume: 3 start-page: 1500002 year: 2015 article-title: Eleven microsatellites in an emerging invader, (Phytolaccaceae), from its native and introduced ranges publication-title: Applications in Plant Sciences – volume: 6 start-page: 102 year: 2016 end-page: 107 article-title: Study on absorption and desorption of publication-title: China Food Additives – volume: 42 start-page: 12 year: 2014 end-page: 16 article-title: Research on the dyeing behavior of wool fabric with natural berry dyes publication-title: Wool Textile Journal – volume: 25 start-page: 57 year: 2009 end-page: 60 article-title: Optimization of total DNA extraction and test of suitable molecular markers in publication-title: Zhongguo Nongxue Tongbao – volume: 17 start-page: 248 year: 2011 end-page: 251 article-title: Review on pharmacological effects and toxicity of phytolaccae publication-title: Chinese Journal of Experimental Traditional Medical Formulae – volume: 21 start-page: 61 year: 2002 end-page: 63 article-title: The status of studies on the distribution and classification of publication-title: Journal of Yanan University – volume: 10 start-page: 564 year: 2010 end-page: 567 article-title: Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows publication-title: Molecular Ecology Resources – volume: 11 start-page: 433 year: 2009 end-page: 438 article-title: Phytolacacinoside A, a new triterpenoid saponin from Roxb publication-title: Journal of Asian Natural Products Research |
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Snippet | Premise of the study:
Phytolacca acinosa (Phytolaccaceae) is a traditional Chinese herb with multiple medicinal uses and is an important pigment source. Due to... |
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SubjectTerms | genetic conservation microsatellite Phytolacca acinosa Phytolacca americana Phytolaccaceae Primer Note |
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Title | Microsatellites for Phytolacca acinosa (Phytolaccaceae), a traditional medicinal herb1 |
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