Microsatellites for Phytolacca acinosa (Phytolaccaceae), a traditional medicinal herb1
Premise of the study: Phytolacca acinosa (Phytolaccaceae) is a traditional Chinese herb with multiple medicinal uses and is an important pigment source. Due to excessive human harvesting, the population numbers and sizes have decreased dramatically. Methods and Results: Using an enriched genomic lib...
Saved in:
| Published in | Applications in plant sciences Vol. 5; no. 10 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Botanical Society of America
19.10.2017
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Premise of the study:
Phytolacca acinosa (Phytolaccaceae) is a traditional Chinese herb with multiple medicinal uses and is an important pigment source. Due to excessive human harvesting, the population numbers and sizes have decreased dramatically.
Methods and Results:
Using an enriched genomic library, we developed and characterized 15 microsatellite primers for P. acinosa, 13 of which were polymorphic. The number of alleles varied from two to seven. The observed heterozygosity and expected heterozygosity per locus ranged from 0.267 to 1.000 and 0.331 to 0.743, respectively. All of the primers that were developed were also successfully applied in P. americana.
Conclusions:
These markers should be useful in probing the genetic diversity, genetic structure, and mating systems of P. acinosa, which could provide information about protecting and sustainably harvesting this species. |
|---|---|
| AbstractList | Premise of the study:
Phytolacca acinosa (Phytolaccaceae) is a traditional Chinese herb with multiple medicinal uses and is an important pigment source. Due to excessive human harvesting, the population numbers and sizes have decreased dramatically.
Methods and Results:
Using an enriched genomic library, we developed and characterized 15 microsatellite primers for P. acinosa, 13 of which were polymorphic. The number of alleles varied from two to seven. The observed heterozygosity and expected heterozygosity per locus ranged from 0.267 to 1.000 and 0.331 to 0.743, respectively. All of the primers that were developed were also successfully applied in P. americana.
Conclusions:
These markers should be useful in probing the genetic diversity, genetic structure, and mating systems of P. acinosa, which could provide information about protecting and sustainably harvesting this species. |
| Author | Cheng, Yueqin Li, Pingping Zhang, Jianhang Yan, Fengming Wang, Hongwei Yang, Yi |
| Author_xml | – sequence: 1 givenname: Yueqin surname: Cheng fullname: Cheng, Yueqin – sequence: 2 givenname: Pingping surname: Li fullname: Li, Pingping – sequence: 3 givenname: Yi surname: Yang fullname: Yang, Yi – sequence: 4 givenname: Jianhang surname: Zhang fullname: Zhang, Jianhang – sequence: 5 givenname: Fengming surname: Yan fullname: Yan, Fengming – sequence: 6 givenname: Hongwei surname: Wang fullname: Wang, Hongwei email: whwcas@163.com |
| BookMark | eNpVUEtLAzEYDFKxtfbmD8hRwa15Z3MRSvEFFQs-riGbZG0k3V12V6X_3pQWH6dvmJlvGOYYDKq68gCcYjSlkpJL0zTdFEuEEMkPwIhgkWeIcTT4g4dg0nXvyYLyRCl2BIZEYaQUzkfg9SHYtu5M72MMve9gWbdwudr0dTTWGmhsqJIMz345640_v4AG9q1xoQ91ZSJcexeSNaGVbwt8Ag5LEzs_2d8xeLm5fp7fZYvH2_v5bJE1mKo889blgpauFNQxJL0wTDqGBSeE8lKVrhBWWUwK6Th3pMBO2oJwSalShiXTGFztcpuPIlWwvkqlom7asDbtRtcm6P9KFVb6rf7UXAimBE0BfBfwFaLf_DxipLf76u2-er-vni2f6B7TbxLucsQ |
| ContentType | Journal Article |
| Copyright | 2017 The Author(s) 2017 Cheng et al. Published by the Botanical Society of America 2017 |
| Copyright_xml | – notice: 2017 The Author(s) – notice: 2017 Cheng et al. Published by the Botanical Society of America 2017 |
| DBID | 24P WIN 5PM |
| DOI | 10.3732/apps.1700028 |
| DatabaseName | Wiley-Blackwell Open Access Collection Wiley-Blackwell Backfiles (Open access) PubMed Central (Full Participant titles) |
| DatabaseTitleList | |
| Database_xml | – sequence: 1 dbid: 24P name: Wiley-Blackwell Open Access Collection url: https://authorservices.wiley.com/open-science/open-access/browse-journals.html sourceTypes: Publisher |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Botany |
| EISSN | 2168-0450 |
| EndPage | n/a |
| ExternalDocumentID | APS31700028 |
| Genre | article |
| GrantInformation_xml | – fundername: National Natural Science Foundation of China funderid: 31670389 |
| GroupedDBID | 0R~ 1OC 24P 5VS 8FE 8FH AAEFD AAHBH AAHHS AAPSS ABDBF ACCFJ ACGFO ACGFS ACXQS ADBBV ADEHT ADHSS ADKYN ADRAZ ADRYA ADZMN ADZOD AEEZP AEGXH AENEX AEPYG AEQDE AFKRA AFNWH AIAGR AIWBW AJBDE AKPMI ALMA_UNASSIGNED_HOLDINGS ALUQN AOIJS AVUZU BBNVY BCNDV BENPR BHPHI CCPQU EBS EJD GROUPED_DOAJ GX1 H13 HCIFZ HYE IAO IGS IPNFZ KQ8 LK8 M48 M7P M~E O9- OK1 PIMPY PQ0 PROAC RBO RIG RNS ROL RPM RZN TBO WIN 5PM |
| ID | FETCH-LOGICAL-p1398-ecd863fdf63d407e6a47d41652235f9fdb6c9c12b7d55d2b1d7cb2573399a4223 |
| IEDL.DBID | RPM |
| ISSN | 2168-0450 |
| IngestDate | Tue Sep 17 21:06:22 EDT 2024 Sat Aug 24 00:53:19 EDT 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 10 |
| Language | English |
| License | Attribution-NonCommercial-ShareAlike This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC-SA 4.0), which permits unrestricted noncommercial use and redistribution provided that the original author and source are credited and the new work is distributed under the same license as the original. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-p1398-ecd863fdf63d407e6a47d41652235f9fdb6c9c12b7d55d2b1d7cb2573399a4223 |
| Notes | This work was supported by the National Natural Science Foundation of China (31670389). |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664963/ |
| PMID | 29109918 |
| PageCount | 3 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_5664963 wiley_primary_10_3732_apps_1700028_APS31700028 |
| PublicationCentury | 2000 |
| PublicationDate | 20171019 |
| PublicationDateYYYYMMDD | 2017-10-19 |
| PublicationDate_xml | – month: 10 year: 2017 text: 20171019 day: 19 |
| PublicationDecade | 2010 |
| PublicationTitle | Applications in plant sciences |
| PublicationYear | 2017 |
| Publisher | Botanical Society of America |
| Publisher_xml | – name: Botanical Society of America |
| References | 2009; 11 1990; 2 2010; 10 2009; 25 2016; 6 2000; 18 2015; 3 2011; 17 2002; 21 2014; 42 |
| References_xml | – volume: 18 start-page: 233 year: 2000 end-page: 234 article-title: An economic method for the fluorescent labeling of PCR fragments publication-title: Nature Biotechnology – volume: 2 start-page: 13 year: 1990 end-page: 16 article-title: Effects of polysaccharides I on cytotoxicity of macrophages and its production of tumor necrosis factor and interleukin 1 publication-title: Chinese Journal of Cancer Research – volume: 3 start-page: 1500002 year: 2015 article-title: Eleven microsatellites in an emerging invader, (Phytolaccaceae), from its native and introduced ranges publication-title: Applications in Plant Sciences – volume: 6 start-page: 102 year: 2016 end-page: 107 article-title: Study on absorption and desorption of publication-title: China Food Additives – volume: 42 start-page: 12 year: 2014 end-page: 16 article-title: Research on the dyeing behavior of wool fabric with natural berry dyes publication-title: Wool Textile Journal – volume: 25 start-page: 57 year: 2009 end-page: 60 article-title: Optimization of total DNA extraction and test of suitable molecular markers in publication-title: Zhongguo Nongxue Tongbao – volume: 17 start-page: 248 year: 2011 end-page: 251 article-title: Review on pharmacological effects and toxicity of phytolaccae publication-title: Chinese Journal of Experimental Traditional Medical Formulae – volume: 21 start-page: 61 year: 2002 end-page: 63 article-title: The status of studies on the distribution and classification of publication-title: Journal of Yanan University – volume: 10 start-page: 564 year: 2010 end-page: 567 article-title: Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows publication-title: Molecular Ecology Resources – volume: 11 start-page: 433 year: 2009 end-page: 438 article-title: Phytolacacinoside A, a new triterpenoid saponin from Roxb publication-title: Journal of Asian Natural Products Research |
| SSID | ssj0000816894 |
| Score | 2.0892954 |
| Snippet | Premise of the study:
Phytolacca acinosa (Phytolaccaceae) is a traditional Chinese herb with multiple medicinal uses and is an important pigment source. Due to... |
| SourceID | pubmedcentral wiley |
| SourceType | Open Access Repository Publisher |
| SubjectTerms | genetic conservation microsatellite Phytolacca acinosa Phytolacca americana Phytolaccaceae Primer Note |
| SummonAdditionalLinks | – databaseName: Scholars Portal Open Access Journals dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NS8MwFA9zevAifuL8IgcPCnbYJE2bg8gUxxAmA53sVpImwYF0c5vg_nvfa-vH1JO30DSEvl_S_F7f6-8RcuydVIYrEyihWCC8doEJeRw4x-MEf13MDEZ0u3ey0xe3g2hQIx_VRisDTv907bCeVH_y3Hx7mV_Chr9AjzPmDEO90ybqzMFZuUSWmeAC13q3IvrFOxnLSyhRZr7_GoSKwApDRFj642du5HfOWhw67XWyVrFF2irh3SA1l2-SlasRMLr5FnnsYjbdVBeimkAZKRBQ2nuaz-CZwGRUZ8McuunJ17XMaXd6RjWFWe2w_A5Iq_g6tABAE26Tfvvm4boTVHUSgjHwtyRwmU0k99ZLbsE_c1KL2ALRAmrFI6-8NTJTWchMbKPIMhNaQIChEKJSWsBNO6Sej3K3S6hOLEtk4s8dOFqCS62dyqRXQCuYtnHSIPGCedJxqYmRokr1Yk8-fCrUqoEvCtjlDdIsDPk5ArwPBCJFINIKiLTVu-dVe-_fU-2TVYaHL-adqANSn01e3SFQh5k5KlbFOzYGxZQ priority: 102 providerName: Scholars Portal – databaseName: Wiley-Blackwell Open Access Collection dbid: 24P link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1LTwMhECZaPXgxPmN9hYMHTUS7wLJwrMamMalpojW9bWCBtJftxtZD_70Du6ltPHmbLAubDMzyzTB8g9CNd0IZpgxRXFHCvXbEJCwjzrFMhquLhQknuoM30R_x13E6bgJu4S5MzQ-xCrgFy4j_62Dg2sQqJCxjgTRUV9X8IbDLwQ65jXYA2ciwqikfrmIssahErIVIQSCAXjp17nsY4nF9gL8pketQNe41vQO034BE3K1n9RBtufII7T7NAMgtj9HnICTRzXXk0gSkiAF34uFkuQAvFTSFdTEtoRnf_j4rnHZ391hj-Kqd1uE_3ByrgwTzZpITNOq9fDz3SVMegVQA2yRxhZWCeesFs-CWOaF5ZgFfAaJiqVfeGlGoIqEms2lqqUksKJ4G_kOlNIeXTlGrnJXuDGEtLZVC-o4D_4ozobVThfAK0ATVNpNtlG2oJ69qKow8kFNvtpTTSSSpBpjIwbjbqJ7EVQ9wOoL286D9vNF-3h2-s0Y-_2-HC7RHw1YbskzUJWotvr7dFQCFhbmOq-EHM2i52w priority: 102 providerName: Wiley-Blackwell |
| Title | Microsatellites for Phytolacca acinosa (Phytolaccaceae), a traditional medicinal herb1 |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.3732%2Fapps.1700028 https://pubmed.ncbi.nlm.nih.gov/PMC5664963 |
| Volume | 5 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV07T8MwED61hYEF8RTlUXlgAIm01HaceCwVVYXUKuIltsiviEo0raAM_HvOboDCyBJZcSJHd1b8ne_zdwCnhRNSM6kjySWNeKFcpLssiZxjSeqPLhrtM7qjsRg-8Jun-KkG8ddZmEDaN3rSLl-m7XLyHLiV86npfPHEOtmojxCE48Tp1KGeMLYSooffr68kIfmS5M4SRn0q-K3tdehwLfXiv9Jng3yVj780yFV4GtaXwRZsVsCQ9JYfsA01V-7A-tUMwdvHLjyOPHHuTQX9TESHBLEmyZ4_FhiZonWIMpMSu8nZzz3jlDu_IIrgqHay3PIjVSodW-gr3d2Dh8H1fX8YVSURojlCtTRyxqaCFbYQzGIo5oTiiUVMhSiKxYUsrBZGmi7ViY1jS3XXorGp1zyUUnF8aB8a5ax0B0BUamkq0uLSYUzFmVDKSSMKiQiCKpukTUh-mSefL-Uvci9I_bsH_RSEqSu_NKEdDPn9BgYa3hG5d0ReOSLvZXesah_-e6gj2KB-nfUUE3kMjcXruztBlLDQLahTnrVg7ep6nN22QqyN1xFPW2G-fAJXBcLZ |
| link.rule.ids | 230,315,733,786,790,870,891,11589,24346,27955,27956,46085,46509,50847,50956,53825,53827 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3JTsMwEB1BQYILO6KsPnAAiaTUTpz4CIiqLEWVgKq3yFvUCggVbQ_w9YyTlKU3uFlxojgzk_hN5vkZ4DC1XCgmlCcCQb0gldZTdRZ51rIodksXtXIV3dYdbz4G192wOwPhZC1MTtrXqu9nzy9-1u_l3MrBi65NeGK1dusCIUiAgVObhTl8X2n4I0nPP8BuLwkRFDR3FjHqisFD3ynR4Wzq5H-Fqwe5fT6miZA_AWo-wzSWoTMZW0EsefLHI-XrjynZxj8PfgWWSsxJzoruVZix2RrMn78iLnxfh07LcfKGMpfmROBJEMaSdu99hEkvGp5I3c-wmxx9H9NW2uMTIgk-jukXfxNJWaXHFoaBqm_AY-Py4aLplbsteANEgbFntYk5S03KmcEsz3IZRAbhGgI0FqYiNYproetURSYMDVV1g36kTk5RCBngSZtQyV4zuwVExobGPE5PLaZrAeNSWqF5KhCcUGmiuArRL7sng0JZI3Fa17970Iq55nVptSr4uYe-rsAcxnk4cR5OSg8nZ-17Vra3_32rA1hoPrRuk9uru5sdWKRuOndMFrELldHb2O4hGBmp_Tz0PgF3--CW |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3JTsMwELXYhLiwI8rqAweQSErt1ImPZanYWlViEeISeVUjIFSQHsrXM05SaHvszYodJZ5x4jeZlzcIHVnDuKRcejzgxAusMJ6s0dAzhoaR-3VRSZfRbbXZ9VNw-1J_GSn1lZP2lUz89P3DT5Nuzq3sfajqkCdW7bQuAIIEsHCqPW2rs2genlkSjgTq-UvY1ZPgQUF1pyElLiH87Ts1OthRnQQwdzkhV-tjkgw5ClLzXaa5gl6H91eQS978fiZ99TMh3TjVBFbRcok9caMYsoZmTLqOFs4_AR8ONtBzy3HzvkUu0QkAFAOcxZ3uIIPgFxyAhUpS6MbH_8eUEebkFAsMU9JJ8VURl9l6aMFykLVN9NS8ery49sqqC14P0GDkGaUjRq22jGqI9gwTQagBtgFQo3XLrZZMcVUjMtT1uiaypsGfxMkqci4CGLSF5tLP1GwjLCJNIhbZMwNhW0CZEIYrZjmAFCJ0GFVQOGb7uFcobMRO83q8ByyZa1-XlqsgP_fS3xkQyzgvx87LcenluNF5oGV7Z-pLHaLFzmUzvr9p3-2iJeJ2dUdo4XtoLvvqm33AJJk8yFffL5TG4xY |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Microsatellites+for+Phytolacca+acinosa+%28Phytolaccaceae%29%2C+a+traditional+medicinal+herb1&rft.jtitle=Applications+in+plant+sciences&rft.au=Cheng%2C+Yueqin&rft.au=Li%2C+Pingping&rft.au=Yang%2C+Yi&rft.au=Zhang%2C+Jianhang&rft.date=2017-10-19&rft.pub=Botanical+Society+of+America&rft.eissn=2168-0450&rft.volume=5&rft.issue=10&rft_id=info:doi/10.3732%2Fapps.1700028&rft_id=info%3Apmid%2F29109918&rft.externalDBID=PMC5664963 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2168-0450&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2168-0450&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2168-0450&client=summon |