Possibility of selective binding of HIV-1 infected cells by immunosorbents

A study was made of a possibility of specific sorption of HIV-1 infected cells with gamma-fraction of AIDS patients' serum containing high titer-specific antibodies, immobilized on the silica matrix (C-3). The laboratory tests were made with the use of the monocyte culture [symbol: see text] ch...

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Published inVestnik Rossiĭskoĭ akademii medits︠i︡nskih nauk no. 3; p. 54
Main Authors Lopukhin, Iu M, Pavlenko, V V, Kulaev, D V, Alekseĭchuk, V S, Karamov, E V, Rudneva, I A, Petrunin, D D, Liukova, T K
Format Journal Article
LanguageRussian
Published Russia (Federation) 1992
Subjects
Acquired Immunodeficiency Syndrome - immunology
Acquired Immunodeficiency Syndrome - metabolism
Cell Count
Cells, Cultured
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
HIV Antibodies - analysis
HIV-1 - immunology
HIV-1 - metabolism
Humans
Immunosorbents
Viral Proteins - metabolism
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Summary:A study was made of a possibility of specific sorption of HIV-1 infected cells with gamma-fraction of AIDS patients' serum containing high titer-specific antibodies, immobilized on the silica matrix (C-3). The laboratory tests were made with the use of the monocyte culture [symbol: see text] chronically infected with HIV-1. In addition to a decrease of the cell count after sorption, there was a decline of the activity of antigen material in the samples (to 43% of the initial). The selectivity rates were 0.92 for immunosorbents and 0.29 for sorbents of the control group, that confirms and increase of the delay of the cells expressing HIV-1 proteins on a column with the immobilized gamma-fraction of AIDS patients' serum. The use of the synthesized immunosorbents in vivo may appear debatable. However, this method can be employed for preparative purposes.
ISSN:0869-6047