Tolerizing mice to human leukocytes: a step toward the production of monoclonal antibodies specific for human dendritic cells

• Published in: Advances in experimental medicine and biology Vol. 329; p. 165
• Main Authors: O'Doherty, U, Swiggard, W J, Inaba, K, Yamaguchi, Y, Kopeloff, I, Bhardwaj, N, Steinman, R M
• Format: Journal Article
• Language: English
• Published: United States 1993
• Subjects: Animals; Animals, Newborn - immunology; Antibodies, Monoclonal - immunology; Cyclophosphamide - pharmacology; Dendritic Cells - immunology; Erythrocytes - immunology; Humans; Immune Tolerance; Immunization; Immunization, Secondary; Male; Mice; Mice, Inbred BALB C - immunology; Mice, Inbred DBA - immunology; Sheep - blood; Species Specificity; T-Lymphocytes - immunology; T-Lymphocytes - transplantation; Transplantation, Heterologous

Despite several attempts to isolate a mAb specific for human dendritic cells, none currently exists. Recent attempts have utilized an improved dendritic cell purification method to prepare immunogens and a rapid two-color flow cytometric screening procedure that allows large numbers of hybridoma sup...